Characterization of human primary chondrocytes of osteoarthritic   cartilage at varying severity

doi:10.3760/cma.j.issn.0366-6999.2011.24.022

CORC > 北京大学 > 工学院

	Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity
	Yin Jing ; Yang Zheng ; Cao Yong-ping ; Ge Zi-gang
刊名	Chinese Medical Journal
	2011
关键词	osteoarthritis chondrocyte differentiation HUMAN ARTICULAR CHONDROCYTES GENE-EXPRESSION IN-VITRO II COLLAGEN PHENOTYPE CULTURE TISSUE VIVO QUANTIFICATION AGGRECANASE-1
DOI	10.3760/cma.j.issn.0366-6999.2011.24.022
英文摘要	Background There is a difficulty in evaluating the in vivo functionality of individual chondrocytes, and there is much heterogeneity among cartilage affected by osteoarthritis (OA). In this study, in vitro cultured chondrocytes harvested from varying stages of degeneration were studied as a projective model to further understand the pathogenesis of osteoarthritis. Methods Cartilage of varying degeneration of end-stage OA was harvested, while cell yield and matrix glycosaminoglycan (GAG) content were measured. Cell morphology, proliferation, and gene expression of collagen type I, II, and X, aggrecan, matrix metalloproteinase 13 (MMP-13), and ADAMTS5 of the acquired chondrocytes were measured during subsequent in vitro culture. Results Both the number of cells and the GAG content increased with increasing severity of OA. Cell spreading area increased and gradually showed spindle-like morphology during in vitro culture. Gene expression of collagen type II, collagen type X as well as GAG decreased with severity of cartilage degeneration, while expression of collagen type I increased. Expression of MMP-13 increased with severity of cartilage degeneration, while expression of ADAMTS-5 remained stable. Expression of collagen type II, X, GAG, and MMP-13 substantially decreased with in vitro culture. Expression of collagen type I increased with in vitro cultures, while expression of ADAMTS 5 remained stable. Conclusions Expression of functional genes such as collagen type II and GAG decreased during severe degeneration of OA cartilage and in vitro dedifferentiation. Gene expression of collagen I and MMP-13 increased with severity of cartilage degeneration. Chin Med J 2011;124(24):4245-4253; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000299061400023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Medicine, General & Internal; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 9; ARTICLE; 24; 4245-4253; 124
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/315696]
专题	工学院
推荐引用方式 GB/T 7714	Yin Jing,Yang Zheng,Cao Yong-ping,et al. Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity[J]. Chinese Medical Journal,2011.
APA	Yin Jing,Yang Zheng,Cao Yong-ping,&Ge Zi-gang.(2011).Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity.Chinese Medical Journal.
MLA	Yin Jing,et al."Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity".Chinese Medical Journal (2011).