Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of   Chondrocytes through Wnt/beta-catenin Signaling Pathway

doi:10.1038/srep05836

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	Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of Chondrocytes through Wnt/beta-catenin Signaling Pathway
	Zhang, Kun ; Guo, Jinsong ; Ge, Zigang ; Zhang, Jue
刊名	scientific reports
	2014
关键词	BETA-CATENIN ARTICULAR-CARTILAGE MEMBRANE PERMEABILIZATION COLLAGEN GENE HUMAN-CELLS IN-VITRO EXPRESSION STIMULATION ACTIVATION APOPTOSIS
DOI	10.1038/srep05836
英文摘要	Nanosecond pulsed electric fields (nsPEFs) characterized by high voltage, low energy and non-thermal effects, have been broadly investigated as a potential tumor therapy; however, little is known about their effects on somatic cells. In this current study, we evaluated effects of nsPEFs on the phenotype of chondrocytes (morphology, glycosaminoglycan (GAG) content, proliferation and gene expression) and explored the mechanisms involved. Our results demonstrated that exposing chondrocytes to nsPEFs led to enhanced proliferation and dedifferentiation, evidenced by the upregulated gene expression of collagen type I (COL I) and downregulated gene expression of Sox9, collagen type II (COL II) and aggrecan (AGG) with activation of the wnt/beta-catenin signaling pathway. Inhibition of the wnt/beta-catenin pathway partially blocked these effects. Thus we concluded that nsPEFs induce dedifferentiation of chondrocytes partially through transient activation of the wnt/beta-catenin signaling pathway.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000339421300017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 0; ARTICLE; gez@pku.edu.cn; zhangjue@pku.edu.cn; 5836; 4
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/315622]
专题	工学院
推荐引用方式 GB/T 7714	Zhang, Kun,Guo, Jinsong,Ge, Zigang,et al. Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of Chondrocytes through Wnt/beta-catenin Signaling Pathway[J]. scientific reports,2014.
APA	Zhang, Kun,Guo, Jinsong,Ge, Zigang,&Zhang, Jue.(2014).Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of Chondrocytes through Wnt/beta-catenin Signaling Pathway.scientific reports.
MLA	Zhang, Kun,et al."Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of Chondrocytes through Wnt/beta-catenin Signaling Pathway".scientific reports (2014).