Bioelectrochemistry of hemoglobin immobilized on a sodium   alginate-multiwall carbon nanotubes composite film

doi:10.1016/j.bios.2008.12.004

CORC > 北京大学 > 工学院

	Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film
	Zhao, H. Y. ; Zheng, W. ; Meng, Z. X. ; Zhou, H. M. ; Xu, X. X. ; Li, Z. ; Zheng, Y. F.
刊名	生物传感器与生物电子学
	2009
关键词	Sodium alginate-multiwall carbon nanotubes composite film Hemoglobin Direct electron transfer Electrocatalysis Biosensor DIRECT ELECTRON-TRANSFER HYDROGEN-PEROXIDE BIOSENSOR BY-LAYER FILMS BIOMEMBRANE-LIKE FILMS DIRECT ELECTROCHEMISTRY HEME-PROTEINS AMPEROMETRIC BIOSENSORS CACO3 NANOPARTICLES CLAY NANOPARTICLES GLUCOSE-OXIDASE
DOI	10.1016/j.bios.2008.12.004
英文摘要	This study described the bioelectrochemistry of hemoglobin (Hb) at multiwall carbon nanotubes (MWCNTs) noncovalently functionalized with biopolymers of sodium alginate (SA). The characteristics of Hb/SA-MWCNTs composite film were studied by using FT-IR spectroscopy, UV-vis spectroscopy and electrochemical methods. Hb immobilized on SA-MWCNTs composite film retained its native secondary structure, achieved direct electron transfer with the apparent heterogeneous electron transfer rate constant (k(s)) of (9.54 +/- 0.883)s(-1) and showed excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide. The amperometric response of the biosensor varied linearly with the H(2)O(2) concentration ranging from 40 to 200 mu M, with a detection limit of 16.41 X 10(-6) M (S/N = 3) and the good long-term stability. Finally, we applied this proposed method to investigate the concentration of hydrogen peroxide in real samples. (C) 2008 Elsevier B.V. All rights reserved.; Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology; SCI(E); EI; 91; ARTICLE; 8; 2352-2357; 24
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/246338]
专题	工学院
推荐引用方式 GB/T 7714	Zhao, H. Y.,Zheng, W.,Meng, Z. X.,et al. Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film[J]. 生物传感器与生物电子学,2009.
APA	Zhao, H. Y..,Zheng, W..,Meng, Z. X..,Zhou, H. M..,Xu, X. X..,...&Zheng, Y. F..(2009).Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film.生物传感器与生物电子学.
MLA	Zhao, H. Y.,et al."Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film".生物传感器与生物电子学 (2009).