Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film | |
Zhao, H. Y. ; Zheng, W. ; Meng, Z. X. ; Zhou, H. M. ; Xu, X. X. ; Li, Z. ; Zheng, Y. F. | |
刊名 | 生物传感器与生物电子学
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2009 | |
关键词 | Sodium alginate-multiwall carbon nanotubes composite film Hemoglobin Direct electron transfer Electrocatalysis Biosensor DIRECT ELECTRON-TRANSFER HYDROGEN-PEROXIDE BIOSENSOR BY-LAYER FILMS BIOMEMBRANE-LIKE FILMS DIRECT ELECTROCHEMISTRY HEME-PROTEINS AMPEROMETRIC BIOSENSORS CACO3 NANOPARTICLES CLAY NANOPARTICLES GLUCOSE-OXIDASE |
DOI | 10.1016/j.bios.2008.12.004 |
英文摘要 | This study described the bioelectrochemistry of hemoglobin (Hb) at multiwall carbon nanotubes (MWCNTs) noncovalently functionalized with biopolymers of sodium alginate (SA). The characteristics of Hb/SA-MWCNTs composite film were studied by using FT-IR spectroscopy, UV-vis spectroscopy and electrochemical methods. Hb immobilized on SA-MWCNTs composite film retained its native secondary structure, achieved direct electron transfer with the apparent heterogeneous electron transfer rate constant (k(s)) of (9.54 +/- 0.883)s(-1) and showed excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide. The amperometric response of the biosensor varied linearly with the H(2)O(2) concentration ranging from 40 to 200 mu M, with a detection limit of 16.41 X 10(-6) M (S/N = 3) and the good long-term stability. Finally, we applied this proposed method to investigate the concentration of hydrogen peroxide in real samples. (C) 2008 Elsevier B.V. All rights reserved.; Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology; SCI(E); EI; 91; ARTICLE; 8; 2352-2357; 24 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/246338] ![]() |
专题 | 工学院 |
推荐引用方式 GB/T 7714 | Zhao, H. Y.,Zheng, W.,Meng, Z. X.,et al. Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film[J]. 生物传感器与生物电子学,2009. |
APA | Zhao, H. Y..,Zheng, W..,Meng, Z. X..,Zhou, H. M..,Xu, X. X..,...&Zheng, Y. F..(2009).Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film.生物传感器与生物电子学. |
MLA | Zhao, H. Y.,et al."Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film".生物传感器与生物电子学 (2009). |
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