Regulation of lipid metabolism in the snow alga Chlamydomonas nivalis in   response to NaCl stress: An integrated analysis by cytomic and lipidomic   approaches

doi:10.1016/j.procbio.2012.04.011

CORC > 北京大学 > 工学院

	Regulation of lipid metabolism in the snow alga Chlamydomonas nivalis in response to NaCl stress: An integrated analysis by cytomic and lipidomic approaches
	Lu, Na ; Wei, Dong ; Jiang, Xiao-Li ; Chen, Feng ; Yang, Shang-Tian
刊名	process biochemistry
	2012
关键词	Chlamydomonas nivalis Cytomic Lipidomic NaCl stress Biomarker MASS-SPECTROMETRY CHROMATOGRAPHY SALINITY BACILLARIOPHYCEAE IDENTIFICATION BIOSYNTHESIS ACCUMULATION GROWTH
DOI	10.1016/j.procbio.2012.04.011
英文摘要	The cultures of the snow alga Chlamydomonas nivalis in the exponential phage were stressed by NaCl (up to 1.5%) for 0 similar to 48 h, followed by Nile Red staining-based cytomic analysis (flow cytometry and confocal laser scanning microscopy). The fluorescent intensities of total lipids, and neutral and polar lipids increased to the maximum within 7 h in the NaCl stressed cells with the highest increase in total lipids by 2-fold (0.75%-NaCl for 7 h), the highest increase in neutral lipids by 68-fold (1%-NaCl for 7h) and the highest increase in polar lipids by 10-fold (1.25%-NaCl for 5 h), respectively. Seven types and 22 kinds of polar lipid molecules were selected and identified as biomarkers by UPLC/Q-TOF-MS-based lipidomic analysis, which demonstrated differences in total lipids between the stress group (0.75%-NaCl for 7 h) and the control. The biological roles of the biomarkers in the alga under NaCl stress were discussed. The integrated approach based on "omics" technologies developed in the present work is validated as a powerful tool to successfully reveal the regulation of lipid metabolism in microalgae in response to stress stimulation. (c) 2012 Elsevier Ltd. All rights reserved,; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical; SCI(E); EI; 0; ARTICLE; 7; 1163-1170; 47
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/232218]
专题	工学院
推荐引用方式 GB/T 7714	Lu, Na,Wei, Dong,Jiang, Xiao-Li,et al. Regulation of lipid metabolism in the snow alga Chlamydomonas nivalis in response to NaCl stress: An integrated analysis by cytomic and lipidomic approaches[J]. process biochemistry,2012.
APA	Lu, Na,Wei, Dong,Jiang, Xiao-Li,Chen, Feng,&Yang, Shang-Tian.(2012).Regulation of lipid metabolism in the snow alga Chlamydomonas nivalis in response to NaCl stress: An integrated analysis by cytomic and lipidomic approaches.process biochemistry.
MLA	Lu, Na,et al."Regulation of lipid metabolism in the snow alga Chlamydomonas nivalis in response to NaCl stress: An integrated analysis by cytomic and lipidomic approaches".process biochemistry (2012).