Microfluidic system for cell mechanics analysis through   dielectrophoresis

doi:10.1016/j.mee.2012.04.023

CORC > 北京大学 > 工学院

	Microfluidic system for cell mechanics analysis through dielectrophoresis
	Guido, Isabella ; Xiong, Chunyang ; Jaeger, Magnus S. ; Duschl, Claus
刊名	微电子工程
	2012
关键词	Cellular stiffness Dielectrophoresis Microsystem On-chip analysis DEFORMATION MECHANOTRANSDUCTION DISEASE
DOI	10.1016/j.mee.2012.04.023
英文摘要	The cellular mechanical properties and changes in cell mechanics are of enormous relevance in many cellular processes and play an important role in the normal or pathological development of cells. We have developed a new technique, the dielectrophoretic stretcher, for analyzing cellular mechanical behavior by stretching cells between microelectrodes through dielectrophoresis. We obtained reliable data that allowed us to unambiguously distinguish between two cell types, one of cancerous origin (MCF-7) and the other from related non-cancerous tissue (MCF-10A). In a second step, this novel technique was integrated into a simple microsystem and was tested by stretching murine cells (Jurkat). These tests verified the compatibility of the dielectrophoretic stretcher with modern microfluidic environments. Our system can additionally be combined with dielectrophoretic manipulation units for aligning, sorting, or positioning cells. It has the potential for automation and, thus, for achieving high throughput. (c) 2012 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000309316300090&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Electrical & Electronic; Nanoscience & Nanotechnology; Optics; Physics, Applied; SCI(E); EI; 6; ARTICLE; 379-382; 97
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/231477]
专题	工学院
推荐引用方式 GB/T 7714	Guido, Isabella,Xiong, Chunyang,Jaeger, Magnus S.,et al. Microfluidic system for cell mechanics analysis through dielectrophoresis[J]. 微电子工程,2012.
APA	Guido, Isabella,Xiong, Chunyang,Jaeger, Magnus S.,&Duschl, Claus.(2012).Microfluidic system for cell mechanics analysis through dielectrophoresis.微电子工程.
MLA	Guido, Isabella,et al."Microfluidic system for cell mechanics analysis through dielectrophoresis".微电子工程 (2012).