A stochastic model for DNA translocation through an electropore

doi:10.1016/j.bbamem.2012.05.025

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	A stochastic model for DNA translocation through an electropore
	Yu, Miao ; Tan, Wenchang ; Lin, Hao
刊名	biochimica et biophysica acta biomembranes
	2012
关键词	Electroporation DNA electrotransfer Fokker-Planck equation Translocation Electrophoresis Diffusion SOLID-STATE NANOPORE MEDIATED GENE DELIVERY LOW-VOLTAGE PULSE ELECTRIC PULSES CELL ELECTROPERMEABILIZATION POLYMER TRANSLOCATION BIOLOGICAL CELLS MAMMALIAN-CELLS BILAYER-MEMBRANES PLASMA-MEMBRANE
DOI	10.1016/j.bbamem.2012.05.025
英文摘要	A 1D Fokker-Planck simulation of DNA translocation through an electropore under finite pulses is presented. This study is motivated by applications relevant to DNA electrotransfer into biological cells via electroporation. The results review important insights. The translocation may occur on two disparate time scales, the electrophoretic time (similar to ms), and the diffusive time (similar to s), depending on the pulse length. Furthermore, a power-law correlation is observed. F-PST similar to(V(m)t(p))(a)/N-b, where F-PST is the final probability of successful translocation, V-m is the transmembrane potential, t(p) is the pulse length, and N is the DNA length in segments. The values for a and b are close to 1 and 1.5, respectively. The simulated results are compared with previous data to interpret the trends. In particular, the diffusive time scale is used to explain the frequency dependence observed in electroporation experiments with uni- and bi-polar pulse trains. The predictions from the current model can be harnessed to help design experiments for the further understanding and quantification of DNA electrotransfer. (C) 2012 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000309081700003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; SCI(E); 10; ARTICLE; 11; 2494-2501; 1818
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/229530]
专题	工学院
推荐引用方式 GB/T 7714	Yu, Miao,Tan, Wenchang,Lin, Hao. A stochastic model for DNA translocation through an electropore[J]. biochimica et biophysica acta biomembranes,2012.
APA	Yu, Miao,Tan, Wenchang,&Lin, Hao.(2012).A stochastic model for DNA translocation through an electropore.biochimica et biophysica acta biomembranes.
MLA	Yu, Miao,et al."A stochastic model for DNA translocation through an electropore".biochimica et biophysica acta biomembranes (2012).