Lipidomic profiling reveals lipid regulation in the snow alga   Chlamydomonas nivalis in response to nitrate or phosphate deprivation

doi:10.1016/j.procbio.2013.02.028

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	Lipidomic profiling reveals lipid regulation in the snow alga Chlamydomonas nivalis in response to nitrate or phosphate deprivation
	Lu, Na ; Wei, Dong ; Chen, Feng ; Yang, Shang-Tian
刊名	process biochemistry
	2013
关键词	Chlamydomonas nivalis Lipdomics UPLC/Q-TOF-MS Nutrient deprivation Biomarker FATTY-ACID MASS-SPECTROMETRY ARABIDOPSIS STARVATION STRESS GALACTOLIPIDS BIOSYNTHESIS METABOLOMICS EXPRESSION DISCOVERY
DOI	10.1016/j.procbio.2013.02.028
英文摘要	The snow alga Chlamydomonas nivalis in the exponential phase was subjected to nitrate or phosphate deprivation for 0-72 h to study its stress responses in lipid profiles analyzed by UPLC/Q-TOF-MS (ultra performance liquid chromatography/quadrupole-time of flight-mass spectrometry). Three clusters were distinguished as the control, nitrate-deprived and phosphate-deprived groups in OPLS-DA (orthogonal projection on latent structure discriminant analysis) score plots based on their lipidome data. Altogether, the lipidomic approach identified twenty-two ions in nitrate-deprived group including nine 1, 2-diacylglyceryl-3-0-4'-(N, N, N-trimethyl)-homoserine (DGTSs), one phosphatidylethanolamine (PE), two monogalactosyldiacylglycerols (MGDGs), four digalactosyldiacylglycerols (DGDGs), three phosphatidylglycerols (PGs), two sulfoquinovosyl-diacylglycerols (SQDGs) and one phosphatidylinositiol (PI), and nineteen ions in phosphate-deprived group including four DGTSs, one PE, one MGDG, seven DGDGs, three PGs, two SQDGs and one PG as "differentiating lipid biomarkers". Moreover, the common and specific biomarkers were found in the two nutrient deprived groups by SUS (shared and unique structure) plot. Biomarkers-based z-score plot and heat map further showed how lipid biomarker expressions deviate from the control. The up- or down-regulation of these lipid biomarkers provided new insights into the lipid metabolism of the snow alga in response to nitrate or phosphate deprivation stress condition. (C) 2013 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000320413900008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical; SCI(E); EI; 9; ARTICLE; 4; 605-613; 48
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/224676]
专题	工学院
推荐引用方式 GB/T 7714	Lu, Na,Wei, Dong,Chen, Feng,et al. Lipidomic profiling reveals lipid regulation in the snow alga Chlamydomonas nivalis in response to nitrate or phosphate deprivation[J]. process biochemistry,2013.
APA	Lu, Na,Wei, Dong,Chen, Feng,&Yang, Shang-Tian.(2013).Lipidomic profiling reveals lipid regulation in the snow alga Chlamydomonas nivalis in response to nitrate or phosphate deprivation.process biochemistry.
MLA	Lu, Na,et al."Lipidomic profiling reveals lipid regulation in the snow alga Chlamydomonas nivalis in response to nitrate or phosphate deprivation".process biochemistry (2013).