Species identification of licorice using nrDNA and cpDNA genetic markers

CORC > 新疆生态与地理研究所 > 中国科学院新疆生态与地理研究所 > 中国科学院新疆生态与地理研究所(2010年以前数据)

	Species identification of licorice using nrDNA and cpDNA genetic markers
	Kondo ; Kenji ; Shiba ; Mao ; Yamaji ; Hiroki ; Morota ; Takashi ; Zhengmin ; Cheng ; Huixia ; Pan ; Shoyama ; Yukhiro
刊名	BIOLOGICAL & PHARMACEUTICAL BULLETIN
	2007
卷号	30 期号:8 页码:1497-1502
关键词	NUCLEAR RIBOSOMAL DNA PHYLOGENETIC RELATIONSHIP RBCL SEQUENCES PLANTS CONSTITUENTS
ISSN号	0918-6158
通讯作者	Kondo, K, Tsumura & Co, Bot Raw Mat Res Dept, Ibaraki 3001192, Japan
中文摘要	For the accurate identification of medicinal licorice species, nucleotide sequences of four types of DNA regions were researched for 205 specimens, including three species used as licorice: Glycyrrhiza uralensis, Glycyrrhiza glabra, and Glycyrrhiza inflata. The four DNA regions were the internal transcribed spacer (ITS) on nuclear ribosomal DNA, the rbcL gene, the matK gene, and the trnH-psbA intergenic region on chloroplast DNA (cpDNA). Ten genotypes were consequently recognized as combinations of the sequence data obtained from the four DNA regions. Species-specific genotypes were defined from the frequency of the appearance of species in each genotype and from the phylogenetic relationships of the 10 genotypes. This revealed the possibility of identifying licorice species based on the 10 genotypes. Next, comparison of species identifications by each DNA region suggested that efficient identification of licorice species is possible using the genetic information obtained from the ITS and trnH-psbA intergenic region. Additionally, concerning the phylogenetic relationships of the Glycyrrhiza species used as licorice, it is suggested from the genetic information of the four types of DNA regions that G. glabra is more closely related to G. inflata than to G. uralensis. In the G. uralensis examined, four genotypes were recognized as intra specific variations. The appearance frequency of each genotype in G. uralensis differed according to the area in China. G. uralensis may have expanded its distribution areas from western to eastern China because many licorices with the phylogenetic ancestral genotype were observed in western areas, while many with the derivative genotype were observed in eastern areas.
学科主题	细胞生物学 ; 生物化学与分子生物学
收录类别	SCI
公开日期	2011-08-19
内容类型	期刊论文
源URL	[http://ir.xjlas.org/handle/365004/10312]
专题	新疆生态与地理研究所_中国科学院新疆生态与地理研究所(2010年以前数据)
推荐引用方式 GB/T 7714	Kondo,Kenji,Shiba,et al. Species identification of licorice using nrDNA and cpDNA genetic markers[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2007,30(8):1497-1502.
APA	Kondo.,Kenji.,Shiba.,Mao.,Yamaji.,...&Yukhiro.(2007).Species identification of licorice using nrDNA and cpDNA genetic markers.BIOLOGICAL & PHARMACEUTICAL BULLETIN,30(8),1497-1502.
MLA	Kondo,et al."Species identification of licorice using nrDNA and cpDNA genetic markers".BIOLOGICAL & PHARMACEUTICAL BULLETIN 30.8(2007):1497-1502.