Mutation Breeding of Extracellular Polysaccharide-Producing Microalga Crypthecodinium cohnii by a Novel Mutagenesis with Atmospheric and Room Temperature Plasma

doi:10.3390/ijms16048201

CORC > 北京大学 > 工学院

	Mutation Breeding of Extracellular Polysaccharide-Producing Microalga Crypthecodinium cohnii by a Novel Mutagenesis with Atmospheric and Room Temperature Plasma
	Liu Bin ; Sun Zheng ; Ma Xiaonian ; Yang Bo ; Jiang Yue ; Wei Dong ; Chen Feng
刊名	international journal of molecular sciences
	2015
DOI	10.3390/ijms16048201
英文摘要	Extracellular polysaccharides (EPS) produced by marine microalgae have the potential to be used as antioxidants, antiviral agents, immunomodulators, and anti-inflammatory agents. Although the marine microalga Crypthecodinium cohnii releases EPS during the process of docosahexaenoic acid (DHA) production, the yield of EPS remains relatively low. To improve the EPS production, a novel mutagenesis of C. cohnii was conducted by atmospheric and room temperature plasma (ARTP). Of the 12 mutants obtained, 10 mutants exhibited significantly enhanced EPS yield on biomass as compared with the wild type strain. Among them, mutant M7 was the best as it could produce an EPS volumetric yield of 1.02 g/L, EPS yield on biomass of 0.39 g/g and EPS yield on glucose of 94 mg/g, which were 33.85%, 85.35% and 57.17% higher than that of the wild type strain, respectively. Results of the present study indicated that mutagenesis of the marine microalga C. cohnii by ARTP was highly effective leading to the high-yield production of EPS.; SCI(E); PubMed; 0; ARTICLE; caisanrenju@163.com; zsun@shou.edu.cn; ydchmn@126.com; ly_mikeyang@163.com; jiangyue@tust.edu.cn; fewd304@scut.edu.cn; sfchencoe@pku.edu.cn; 4; 8201-12; 16
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188105]
专题	工学院
推荐引用方式 GB/T 7714	Liu Bin,Sun Zheng,Ma Xiaonian,et al. Mutation Breeding of Extracellular Polysaccharide-Producing Microalga Crypthecodinium cohnii by a Novel Mutagenesis with Atmospheric and Room Temperature Plasma[J]. international journal of molecular sciences,2015.
APA	Liu Bin.,Sun Zheng.,Ma Xiaonian.,Yang Bo.,Jiang Yue.,...&Chen Feng.(2015).Mutation Breeding of Extracellular Polysaccharide-Producing Microalga Crypthecodinium cohnii by a Novel Mutagenesis with Atmospheric and Room Temperature Plasma.international journal of molecular sciences.
MLA	Liu Bin,et al."Mutation Breeding of Extracellular Polysaccharide-Producing Microalga Crypthecodinium cohnii by a Novel Mutagenesis with Atmospheric and Room Temperature Plasma".international journal of molecular sciences (2015).