Polycaprolactone electrospun mesh conjugated with an MSC affinity   peptide for MSC homing in vivo

doi:10.1016/j.biomaterials.2012.01.033

CORC > 北京大学 > 工学院

	Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo
	Shao, Zhenxing ; Zhang, Xin ; Pi, Yanbin ; Wang, Xiaokun ; Jia, Zhuqing ; Zhu, Jingxian ; Dai, Linghui ; Chen, Wenqing ; Yin, Ling ; Chen, Haifeng ; Zhou, Chunyan ; Ao, Yingfang
刊名	biomaterials
	2012
关键词	Phage display Tissue engineering (TE) MSC-homing Peptide-modified polycaprolactone electrospun mesh MESENCHYMAL STEM-CELLS AUTOLOGOUS CHONDROCYTE IMPLANTATION THERAPY POSITION STATEMENT PHAGE DISPLAY INTERNATIONAL-SOCIETY RANDOMIZED-TRIAL STROMAL CELLS MICROFRACTURE BIOMATERIALS REGENERATION
DOI	10.1016/j.biomaterials.2012.01.033
英文摘要	Mesenchymal stem cell (MSC) is a promising cell source candidate in tissue engineering (TE) and regenerative medicine. However, the inability to target MSCs in tissues of interest with high efficiency and engraftment has become a significant barrier for MSC-based therapies. The mobilization and transfer of MSCs to defective/damaged sites in tissues or organs in vivo with high efficacy and efficiency has been a major concern. In the present study, we identified a peptide sequence (E7) with seven amino acids through phage display technology, which has a high specific affinity to bone marrow-derived MSCs. Subsequent analysis suggested that the peptide could efficiently interact specifically with MSCs without any species specificity. Thereafter, E7 was covalently conjugated onto polycaprolactone (PCL) electrospun meshes to construct an "MSC-homing device" for the recruitment of MSCs both in vitro and in vivo. The E7-conjugated PCL electrospun meshes were implanted into a cartilage defect site of rat knee joints, combined with a microfracture procedure to mobilize the endogenous MSCs. After 7 d of implantation, immunofluorescence staining showed that the cells grown into the E7-conjugated PCL electrospun meshes yielded a high positive rate for specific MSC surface markers (CD44, CD90, and CD105) compared with those in arginine-glycine-aspartic acid (RGD)-conjugated PCL electrospun meshes (63.67% vs. 3.03%; 59.37% vs. 2.98%; and 61.45% vs. 3.82%, respectively). Furthermore, the percentage of CD68 positive cells in the E7-conjugated PCL electrospun meshes was much lower than that in the RGD-conjugated PCL electrospun meshes (5.57% vs. 53.43%). This result indicates that E7-conjugated PCL electrospun meshes absorb much less inflammatory cells in vivo than RGD-conjugated PCL electrospun meshes. The results of the present study suggest that the identified E7 peptide sequence has a high specific affinity to MSCs. Covalently conjugating this peptide on the synthetic PCL mesh significantly enhanced the MSC recruitment of PCL in vivo. This method provides a wide range of potential applications in TE. (C) 2012 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000301886300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Biomedical; Materials Science, Biomaterials; SCI(E); EI; PubMed; 28; ARTICLE; 12; 3375-3387; 33
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/161514]
专题	工学院
推荐引用方式 GB/T 7714	Shao, Zhenxing,Zhang, Xin,Pi, Yanbin,et al. Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo[J]. biomaterials,2012.
APA	Shao, Zhenxing.,Zhang, Xin.,Pi, Yanbin.,Wang, Xiaokun.,Jia, Zhuqing.,...&Ao, Yingfang.(2012).Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo.biomaterials.
MLA	Shao, Zhenxing,et al."Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo".biomaterials (2012).