Inactivation of Candida Biofilms by Non-Thermal Plasma and Its   Enhancement for Fungistatic Effect of Antifungal Drugs

doi:10.1371/journal.pone.0040629

CORC > 北京大学 > 工学院

	Inactivation of Candida Biofilms by Non-Thermal Plasma and Its Enhancement for Fungistatic Effect of Antifungal Drugs
	Sun, Yi ; Yu, Shuang ; Sun, Peng ; Wu, Haiyan ; Zhu, Weidong ; Liu, Wei ; Zhang, Jue ; Fang, Jing ; Li, Ruoyu
刊名	plos one
	2012
关键词	PRESSURE AIR PLASMA INVASIVE CANDIDIASIS ALBICANS BIOFILMS HYDROGEN-PEROXIDE SINGLET OXYGEN RESISTANCE COAGULATION EPIDEMIOLOGY MICROJET BACTERIA
DOI	10.1371/journal.pone.0040629
英文摘要	We investigated the antifungal effect of non-thermal plasma, as well as its combination with common antifungal drugs, against Candida biofilms. A direct current atmospheric pressure He/O-2 (2%) plasma microjet (PMJ) was used to treat Candida biofilms in a 96-well plate. Inactivation efficacies of the biofilms were evaluated by XTT assay and counting colony forming units (CFUs). Morphological properties of the biofilms were evaluated by Scanning Electron Microscope (SEM). The sessile minimal inhibitory concentrations (SMICs) of fluconazole, amphotericin B, and caspofungin for the biofilms were also tested. Electron Spin Resonance (ESR) spectroscopy was used to detect the reactive oxygen species (ROS) generated directly and indirectly by PMJ. The Candida biofilms were completely inactivated after 1 min PMJ treatment, where severely deformed fungal elements were observed in SEM images. The SMICs of the tested antifungal drugs for the plasma-treated biofilms were decreased by 2-6 folds of dilution, compared to those of the untreated controls. ROS such as hydroxyl radical ((OH)-O-center dot), superoxide anion radical (O-center dot(2)) and singlet molecular oxygen (O-1(2)) were detected by ESR. We hence conclude that He/O-2 (2%) plasma alone, as well as in combination with common antifungal drugs, is able to inactivate Candida biofilms rapidly. The generation of ROS is believed to be one of the underlying mechanisms for the fungicidal activity; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000306355500064&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 9; ARTICLE; 7; e40629; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/160114]
专题	工学院
推荐引用方式 GB/T 7714	Sun, Yi,Yu, Shuang,Sun, Peng,et al. Inactivation of Candida Biofilms by Non-Thermal Plasma and Its Enhancement for Fungistatic Effect of Antifungal Drugs[J]. plos one,2012.
APA	Sun, Yi.,Yu, Shuang.,Sun, Peng.,Wu, Haiyan.,Zhu, Weidong.,...&Li, Ruoyu.(2012).Inactivation of Candida Biofilms by Non-Thermal Plasma and Its Enhancement for Fungistatic Effect of Antifungal Drugs.plos one.
MLA	Sun, Yi,et al."Inactivation of Candida Biofilms by Non-Thermal Plasma and Its Enhancement for Fungistatic Effect of Antifungal Drugs".plos one (2012).