Engineering of an endogenous phytoene desaturase gene as a dominant   selectable marker for Chlamydomonas reinhardtii transformation and   enhanced biosynthesis of carotenoids

doi:10.1016/j.procbio.2013.04.020

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	Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids
	Liu, Jin ; Gerken, Henri ; Huang, Junchao ; Chen, Feng
刊名	process biochemistry
	2013
关键词	Chlamydomonas reinhardtii Carotenoids Norflurazon Phytoene desaturase Selectable marker Site-directed mutation NUCLEAR TRANSFORMATION CHLORELLA-ZOFINGIENSIS HERBICIDE NORFLURAZON FOREIGN GENE EXPRESSION RESISTANCE MUTANTS PLANTS PCR
DOI	10.1016/j.procbio.2013.04.020
英文摘要	A phytoene desaturase (PDS) gene was cloned and characterized from the unicellular green microalga Chlamydomonas reinhardtii. Functional complementation analysis revealed C reinhardtii PDS (CrPDS) catalyzes the conversion of phytoene to the colored carotenoid zeta-carotene. A single amino acid substitution, L505F, enhanced its desaturation activity by 29%, as indicated by an in vitro enzymatic assay. In addition, CrPDS-L505F exhibited 27.7-fold higher resistance to the herbicide norflurazon. Glass bead-mediated delivery displayed a high transformation efficiency of C reinhardtii with CrPDS-L505F, demonstrating clearly that the engineered endogenous CrPDS is a dominant selectable marker for C. reinhardtii and possibly for other green algae. Furthermore, the expression of PDS could enhance the intracellular carotenoid accumulation of transformants, opening up the possibility of engineering the carotenogenic pathway for improved carotenoid production in microalgae. (C) 2013 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000321423600006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical; SCI(E); EI; 8; ARTICLE; 5-6; 788-795; 48
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/154488]
专题	工学院
推荐引用方式 GB/T 7714	Liu, Jin,Gerken, Henri,Huang, Junchao,et al. Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids[J]. process biochemistry,2013.
APA	Liu, Jin,Gerken, Henri,Huang, Junchao,&Chen, Feng.(2013).Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids.process biochemistry.
MLA	Liu, Jin,et al."Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids".process biochemistry (2013).