Binding of phenanthrene to extracellular polymeric substances (EPS) from aerobic activated sludge: A fluorescence study

CORC > 新疆生态与地理研究所 > 中国科学院新疆生态与地理研究所 > 中国科学院新疆生态与地理研究所(2010年以前数据)

	Binding of phenanthrene to extracellular polymeric substances (EPS) from aerobic activated sludge: A fluorescence study
	Pan ; Xiangliang ; Liu ; Jing ; Zhang ; Daoyong
刊名	COLLOIDS AND SURFACES B-BIOINTERFACES
	2010
卷号	80 期号:1 页码:103-106
关键词	DISSOLVED ORGANIC-MATTER BOVINE SERUM-ALBUMIN AROMATIC-HYDROCARBONS PAHS HEAVY-METALS BIOSORPTION PROPERTIES ANAEROBIC SLUDGE CADMIUM COMPLEXATION SPECTROSCOPY LEAD
ISSN号	0927-7765
通讯作者	Zhang, DY, Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, 46 Guanshui Rd, Guiyang 550002, Peoples R China
中文摘要	Extracellular polymeric substances (EPS) are the essential components of activated sludge for removal of pollutants from wastewater. Limited information is available on the binding constants and binding mode of organic pollutants to EPS. In the present study, binding of phenanthrene (PHE) to extracellular polymeric substances (EPS) from aerobic activated sludge was investigated using fluorescence spectroscopy. Two protein-like fluorescence peaks (Ex/Em = 225 nm/340 nm for peak A: Ex/Em = 275-280 nm/338 nm for peak B) were identified in the three-dimensional excitation-emission matrix (3DEEM) fluorescence spectroscopy of the EPS. The fluorophores in EPS were clearly quenched by PHE and the quenching processes were static. The quenching constants (In K-d) were in the range of 11.27-13.82 M-1 and the binding constants (log K-b) in the range of 6.11-8.98 M-1. The binding site number increased with increasing temperature. The corresponding thermodynamic parameters Delta G, Delta H and Delta S were calculated. The interaction of the fluorophores in EPS and PHE is spontaneous and exothermic. The binding of EPS with PHE is dominated by the hydrophobic interactions. Fluorophore A has stronger hydrophobic interaction with PHE than fluorophore B. (c) 2010 Elsevier B.V. All rights reserved.
学科主题	生物化学与分子生物学
收录类别	SCI
公开日期	2011-08-19
内容类型	期刊论文
源URL	[http://ir.xjlas.org/handle/365004/9936]
专题	新疆生态与地理研究所_中国科学院新疆生态与地理研究所(2010年以前数据)
推荐引用方式 GB/T 7714	Pan,Xiangliang,Liu,et al. Binding of phenanthrene to extracellular polymeric substances (EPS) from aerobic activated sludge: A fluorescence study[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2010,80(1):103-106.
APA	Pan,Xiangliang,Liu,Jing,Zhang,&Daoyong.(2010).Binding of phenanthrene to extracellular polymeric substances (EPS) from aerobic activated sludge: A fluorescence study.COLLOIDS AND SURFACES B-BIOINTERFACES,80(1),103-106.
MLA	Pan,et al."Binding of phenanthrene to extracellular polymeric substances (EPS) from aerobic activated sludge: A fluorescence study".COLLOIDS AND SURFACES B-BIOINTERFACES 80.1(2010):103-106.