Cell Spreading and Proliferation in Response to the Composition and   Mechanics of Engineered Fibrillar Extracellular Matrices

doi:10.1002/bit.24921

CORC > 北京大学 > 工学院

	Cell Spreading and Proliferation in Response to the Composition and Mechanics of Engineered Fibrillar Extracellular Matrices
	Chen, Antony K. ; Delrio, Frank W. ; Peterson, Alexander W. ; Chung, Koo-Hyun ; Bhadiraju, Kiran ; Plant, Anne L.
刊名	biotechnology and bioengineering
	2013
关键词	fibronectin collagen combined matrix stiffness extracellular matrix THIN-FILMS I COLLAGEN ADHESION RECEPTORS BINDING-SITES FIBRONECTIN SHAPE VITRONECTIN MICROSCOPY STIFFNESS FORCES
DOI	10.1002/bit.24921
英文摘要	The extracellular matrix (ECM) consists of a complex mixture of biochemical and physical stimuli that together regulate cell behavior. In this study, we engineer a model ECM consisting of fibrillar Type-1 collagen plus fibronectin that allows systematic examination of the effects of matrix composition and mechanics on cells. On this combined protein matrix, cells exhibit intermediate degrees of spreading and proliferation compared to their responses on collagen or fibronectin alone. Adhesion to the combination matrix could be blocked by peptides containing the sequence arginine-glycine-aspartic acid (RGD) and by antibodies against 1 integrin, suggesting cell-matrix engagement was mediated by a combination of integrin receptors that recognize fibronectin and collagen. Regardless of integrin engagement, cells were sensitive to the mechanical properties of the combination ECM, suggesting that cells could process biochemical and mechanical cues simultaneously and independently. Biotechnol. Bioeng. 2013;110: 2731-2741. (c) 2013 Wiley Periodicals, Inc.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000327725400018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biotechnology & Applied Microbiology; SCI(E); EI; 7; ARTICLE; 10; 2731-2741; 110
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/154406]
专题	工学院
推荐引用方式 GB/T 7714	Chen, Antony K.,Delrio, Frank W.,Peterson, Alexander W.,et al. Cell Spreading and Proliferation in Response to the Composition and Mechanics of Engineered Fibrillar Extracellular Matrices[J]. biotechnology and bioengineering,2013.
APA	Chen, Antony K.,Delrio, Frank W.,Peterson, Alexander W.,Chung, Koo-Hyun,Bhadiraju, Kiran,&Plant, Anne L..(2013).Cell Spreading and Proliferation in Response to the Composition and Mechanics of Engineered Fibrillar Extracellular Matrices.biotechnology and bioengineering.
MLA	Chen, Antony K.,et al."Cell Spreading and Proliferation in Response to the Composition and Mechanics of Engineered Fibrillar Extracellular Matrices".biotechnology and bioengineering (2013).