Cell Spreading and Proliferation in Response to the Composition and Mechanics of Engineered Fibrillar Extracellular Matrices | |
Chen, Antony K. ; Delrio, Frank W. ; Peterson, Alexander W. ; Chung, Koo-Hyun ; Bhadiraju, Kiran ; Plant, Anne L. | |
刊名 | biotechnology and bioengineering
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2013 | |
关键词 | fibronectin collagen combined matrix stiffness extracellular matrix THIN-FILMS I COLLAGEN ADHESION RECEPTORS BINDING-SITES FIBRONECTIN SHAPE VITRONECTIN MICROSCOPY STIFFNESS FORCES |
DOI | 10.1002/bit.24921 |
英文摘要 | The extracellular matrix (ECM) consists of a complex mixture of biochemical and physical stimuli that together regulate cell behavior. In this study, we engineer a model ECM consisting of fibrillar Type-1 collagen plus fibronectin that allows systematic examination of the effects of matrix composition and mechanics on cells. On this combined protein matrix, cells exhibit intermediate degrees of spreading and proliferation compared to their responses on collagen or fibronectin alone. Adhesion to the combination matrix could be blocked by peptides containing the sequence arginine-glycine-aspartic acid (RGD) and by antibodies against 1 integrin, suggesting cell-matrix engagement was mediated by a combination of integrin receptors that recognize fibronectin and collagen. Regardless of integrin engagement, cells were sensitive to the mechanical properties of the combination ECM, suggesting that cells could process biochemical and mechanical cues simultaneously and independently. Biotechnol. Bioeng. 2013;110: 2731-2741. (c) 2013 Wiley Periodicals, Inc.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000327725400018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biotechnology & Applied Microbiology; SCI(E); EI; 7; ARTICLE; 10; 2731-2741; 110 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/154406] ![]() |
专题 | 工学院 |
推荐引用方式 GB/T 7714 | Chen, Antony K.,Delrio, Frank W.,Peterson, Alexander W.,et al. Cell Spreading and Proliferation in Response to the Composition and Mechanics of Engineered Fibrillar Extracellular Matrices[J]. biotechnology and bioengineering,2013. |
APA | Chen, Antony K.,Delrio, Frank W.,Peterson, Alexander W.,Chung, Koo-Hyun,Bhadiraju, Kiran,&Plant, Anne L..(2013).Cell Spreading and Proliferation in Response to the Composition and Mechanics of Engineered Fibrillar Extracellular Matrices.biotechnology and bioengineering. |
MLA | Chen, Antony K.,et al."Cell Spreading and Proliferation in Response to the Composition and Mechanics of Engineered Fibrillar Extracellular Matrices".biotechnology and bioengineering (2013). |
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