Point-of-Care Multiplexed Assays of Nucleic Acids Using   Microcapillary-based Loop-Mediated Isothermal Amplification

doi:10.1021/ac5014332

CORC > 北京大学 > 工学院

	Point-of-Care Multiplexed Assays of Nucleic Acids Using Microcapillary-based Loop-Mediated Isothermal Amplification
	Zhang, Yi ; Zhang, Lu ; Sun, Jiashu ; Liu, Yulei ; Ma, Xingjie ; Cui, Shangjin ; Ma, Liying ; Xi, Jianzhong Jeff ; Jiang, Xingyu
刊名	analytical chemistry
	2014
关键词	POLYMERASE-CHAIN-REACTION CAPILLARY SENSOR ARRAY REVERSE-TRANSCRIPTION MICROFLUIDIC SYSTEMS RAPID DETECTION DNA EXTRACTION DIGITAL PCR LAMP DEVICE ELECTROPHORESIS
DOI	10.1021/ac5014332
英文摘要	This report demonstrates a straightforward, robust, multiplexed and point-of-care microcapillary-based loop-mediated isothermal amplification (cLAMP) for assaying nucleic acids. This assay integrates capillaries (glass or plastic) to introduce and house sample/reagents, segments of water droplets to prevent contamination, pocket warmers to provide heat, and a hand-held flashlight for a visual readout of the fluorescent signal. The cLAMP system allows the simultaneous detection of two RNA targets of human immunodeficiency virus (HIV) from multiple plasma samples, and achieves a high sensitivity of two copies of standard plasmid. As few nucleic acid detection methods can be wholly independent of external power supply and equipment, our cLAMP holds great promise for point-of-care applications in resource-poor settings.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000339227400046&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; SCI(E); EI; 14; ARTICLE; sunjs@nanoctr.cn; xingyujiang@nanoctr.cn; 14; 7057-7062; 86
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/154258]
专题	工学院
推荐引用方式 GB/T 7714	Zhang, Yi,Zhang, Lu,Sun, Jiashu,et al. Point-of-Care Multiplexed Assays of Nucleic Acids Using Microcapillary-based Loop-Mediated Isothermal Amplification[J]. analytical chemistry,2014.
APA	Zhang, Yi.,Zhang, Lu.,Sun, Jiashu.,Liu, Yulei.,Ma, Xingjie.,...&Jiang, Xingyu.(2014).Point-of-Care Multiplexed Assays of Nucleic Acids Using Microcapillary-based Loop-Mediated Isothermal Amplification.analytical chemistry.
MLA	Zhang, Yi,et al."Point-of-Care Multiplexed Assays of Nucleic Acids Using Microcapillary-based Loop-Mediated Isothermal Amplification".analytical chemistry (2014).