Functionalized dendrimer-based delivery of angiotensin type 1 receptor   siRNA for preserving cardiac function following infarction

doi:10.1016/j.biomaterials.2013.02.008

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	Functionalized dendrimer-based delivery of angiotensin type 1 receptor siRNA for preserving cardiac function following infarction
	Liu, Jie ; Gu, Catherine ; Cabigas, E. Bernadette ; Pendergrass, Karl D. ; Brown, Milton E. ; Luo, Ying ; Davis, Michael E.
刊名	biomaterials
	2013
关键词	Dendrimer Cardiomyocyte Gene expression Ischemia-reperfusion CORONARY-ARTERY-DISEASE ISOLATED RAT HEARTS MYOCARDIAL-INFARCTION CARDIOVASCULAR-DISEASE ALDOSTERONE SYSTEM RANDOMIZED TRIAL AT(2) RECEPTORS GENE DELIVERY IN-VITRO BLOCKADE
DOI	10.1016/j.biomaterials.2013.02.008
英文摘要	Cardiovascular disease (CVD) is the leading cause of death throughout the world and much pathology is associated with upregulation of inflammatory genes. Gene silencing using RNA interference is a powerful tool in regulating gene expression, but its application in CVDs has been prevented by the lack of efficient delivery systems. We report here the development of tadpole dendrimeric materials for siRNA delivery in a rat ischemia-reperfusion (IR) model. Angiotensin II (Ang II) type 1 receptor (AT1R), the major receptor that mediates most adverse effects of Ang II, was chosen to be the silencing targeting. Among the three tadpole dendrimers synthesized, the oligo-arginine conjugated dendrimer loaded with siRNA demonstrated effective down-regulation in AT1R expression in cardiomyocytes in vitro. When the dendrimeric material was applied in vivo, the siRNA delivery prevented the increase in AT1R levels and significantly improved cardiac function recovery compared to saline injection or empty dendrimer treated groups after IR injury. These experiments demonstrate a potential treatment-for dysfunction caused by IR injury and may represent an alternative to AT1R blockade. (C) 2013 Elsevier Ltd. All rights reserved.; Engineering, Biomedical; Materials Science, Biomaterials; SCI(E); EI; PubMed; 7; ARTICLE; 14; 3729-3736; 34
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/153940]
专题	工学院
推荐引用方式 GB/T 7714	Liu, Jie,Gu, Catherine,Cabigas, E. Bernadette,et al. Functionalized dendrimer-based delivery of angiotensin type 1 receptor siRNA for preserving cardiac function following infarction[J]. biomaterials,2013.
APA	Liu, Jie.,Gu, Catherine.,Cabigas, E. Bernadette.,Pendergrass, Karl D..,Brown, Milton E..,...&Davis, Michael E..(2013).Functionalized dendrimer-based delivery of angiotensin type 1 receptor siRNA for preserving cardiac function following infarction.biomaterials.
MLA	Liu, Jie,et al."Functionalized dendrimer-based delivery of angiotensin type 1 receptor siRNA for preserving cardiac function following infarction".biomaterials (2013).