Enhanced Sensing of Nucleic Acids with Silicon Nanowire Field Effect   Transistor Biosensors

doi:10.1021/nl302476h

CORC > 北京大学 > 信息科学技术学院

	Enhanced Sensing of Nucleic Acids with Silicon Nanowire Field Effect Transistor Biosensors
	Gao, Anran ; Lu, Na ; Wang, Yuchen ; Dai, Pengfei ; Li, Tie ; Gao, Xiuli ; Wang, Yuelin ; Fan, Chunhai
刊名	nano letters
	2012
关键词	SiNW-FETs biosensor ultrasensitive detection limit LABEL-FREE DETECTION SEQUENCE-SPECIFIC DETECTION ELECTRICAL DETECTION DNA HYBRIDIZATION SENSOR ARRAYS AMPLIFICATION OPTIMIZATION SPECTROSCOPY NANOSENSORS ELECTRODES
DOI	10.1021/nl302476h
英文摘要	Silicon nanowire (SiNW) field effect transistors (FETs) have emerged as powerful sensors for ultrasensitive, direct electrical readout, and label-free biological/chemical detection. The sensing mechanism of SiNW-FET can be understood in terms of the change in charge density at the SiNW surface after hybridization. So far, there have been limited systematic studies on fundamental factors related to device sensitivity to further make clear the overall effect on sensing sensitivity. Here, we present an analytical result for our triangle cross-section wire for predicting the sensitivity of nanowire surface-charge sensors. It was confirmed through sensing experiments that the back-gated SiNW-FET sensor had the highest percentage current response in the subthreshold regime and the sensor performance could be optimized in low buffer ionic strength and at moderate probe concentration. The optimized SiNW-FET nanosensor revealed ultrahigh sensitivity for rapid and reliable detection of target DNA with a detection limit of 0.1 fM and high specificity for single-nucleotide polymorphism discrimination. In our work, enhanced sensing of biological species by optimization of operating parameters and fundamental understanding for SiNW PET detection limit was obtained.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000309615000032&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter; SCI(E); EI; 77; ARTICLE; 10; 5262-5268; 12
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/231216]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Gao, Anran,Lu, Na,Wang, Yuchen,et al. Enhanced Sensing of Nucleic Acids with Silicon Nanowire Field Effect Transistor Biosensors[J]. nano letters,2012.
APA	Gao, Anran.,Lu, Na.,Wang, Yuchen.,Dai, Pengfei.,Li, Tie.,...&Fan, Chunhai.(2012).Enhanced Sensing of Nucleic Acids with Silicon Nanowire Field Effect Transistor Biosensors.nano letters.
MLA	Gao, Anran,et al."Enhanced Sensing of Nucleic Acids with Silicon Nanowire Field Effect Transistor Biosensors".nano letters (2012).