Rational construction of a cellular memory inverter

doi:10.1007/s11432-014-5193-6

CORC > 北京大学 > 信息科学技术学院

	Rational construction of a cellular memory inverter
	Chen Mei ; Zhang Lu ; Xu Jin
刊名	science china information sciences
	2015
关键词	cellular memory inverter lac operon cre recombinase ordinary differential equation (ODE) biological computing system STRAND DISPLACEMENT CASCADES ESCHERICHIA-COLI DNA RECOMBINATION COMPUTATION NETWORKS CIRCUIT BINDING PROTEIN
DOI	10.1007/s11432-014-5193-6
英文摘要	Robust and reliable biological memory computing systems are lacking, although many biological computing systems are implemented. We successfully designed, constructed, and tested a cellular memory inverter in the bacterium Escherichia coli in a predictable way. We selected isopropyl -D-1-thiogalactopyranoside (IPTG) as the input and green fluorescent protein (GFP) as the output. We selected negative regulation of lac operon and excision function of Cre recombinase as computing tools. The GFP can express and produce output without IPTG. The input, IPTG, can induce the expression of Cre recombinase. The Cre recombinase can excise the GFP gene between site lox71 and site lox66, and hence turn off the output. We also selected ordinary differential equations (ODEs) to predict the dynamic behaviors for the precise design and implementation of the inverter. We used sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), colony Polymerase Chain Reaction (PCR), and fluorescent quantitative analysis techniques to verify the function of our inverter. The study provides a new biological memory computing system. The Cre recombinase functions used here should be useful in the construction of biological memory computing systems.; Computer Science, Information Systems; SCI(E); EI; 中国科学引文数据库(CSCD); 1; ARTICLE; zhijin@pku.edu.cn; 1; 1-8; 58
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/217701]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Chen Mei,Zhang Lu,Xu Jin. Rational construction of a cellular memory inverter[J]. science china information sciences,2015.
APA	Chen Mei,Zhang Lu,&Xu Jin.(2015).Rational construction of a cellular memory inverter.science china information sciences.
MLA	Chen Mei,et al."Rational construction of a cellular memory inverter".science china information sciences (2015).