Hybrid microsystem with functionalized silicon substrate and PDMS sample   operating microchannel: A reconfigurable microfluidics scheme

doi:10.1007/s11431-010-3175-5

CORC > 北京大学 > 信息科学技术学院

	Hybrid microsystem with functionalized silicon substrate and PDMS sample operating microchannel: A reconfigurable microfluidics scheme
	Lei YinHua ; Xie Fei ; Wang Wei ; Wang HaiBin ; Li ZhiHong
刊名	science china technological sciences
	2010
关键词	reconfigurable microfluidics hybrid microdevice thin PDMS film FLOW PCR CHIP DEVICE AMPLIFICATION FABRICATION
DOI	10.1007/s11431-010-3175-5
英文摘要	A hybrid microsystem with separately functioned temperature controlling substrate and sample operating fluidic microchannel was developed to demonstrate a reconfigurable microfluidics scheme. The temperature controlling substrate integrated a micro heater and a temperature sensor by using traditional silicon-based micromechanical system (MEMS) technique, which guaranteed high performance and robust reliability for repeatable usage. The sample operating fluidic microchannel was prepared by poly-(dimethylsiloxane) (PDMS) based soft lithography technique, which made it cheap enough for disposable applications. The PDMS microchannel chip was attached to the temperature controlling substrate for reconfigurable thermal applications. A thin PDMS film was used to seal the microchannel and bridge the functionalized substrate and the sample inside the channel, which facilitated heat transferring and prevented sample contaminating the temperature controlling substrate. Demonstrated by a one dimensional thermal resistance model, the thin PDMS film was important for the present reconfiguration applications. Thermal performance of this hybrid microsystem was examined, and the experimental results demonstrated that the chip system could work stably over hours with temperature variation less than 0.1A degrees C. Multiple PDMS microchannel chips were tested on one heating substrate sequentially with a maximum intra-chip temperature difference of 1.0A degrees C. DNA extracted from serum of a chronic hepatitis B virus (HBV) patient was amplified by this hybrid microsystem and the gel electrophoresis result indicated that the present reconfigurable microfluidic scheme worked successfully.; Engineering, Multidisciplinary; Materials Science, Multidisciplinary; SCI(E); EI; 0; ARTICLE; 8; 2272-2277; 53
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/152679]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Lei YinHua,Xie Fei,Wang Wei,et al. Hybrid microsystem with functionalized silicon substrate and PDMS sample operating microchannel: A reconfigurable microfluidics scheme[J]. science china technological sciences,2010.
APA	Lei YinHua,Xie Fei,Wang Wei,Wang HaiBin,&Li ZhiHong.(2010).Hybrid microsystem with functionalized silicon substrate and PDMS sample operating microchannel: A reconfigurable microfluidics scheme.science china technological sciences.
MLA	Lei YinHua,et al."Hybrid microsystem with functionalized silicon substrate and PDMS sample operating microchannel: A reconfigurable microfluidics scheme".science china technological sciences (2010).