Single-cell discrimination based on optical tweezers Raman spectroscopy

doi:10.1007/s11434-013-5721-6

CORC > 北京大学 > 信息科学技术学院

	Single-cell discrimination based on optical tweezers Raman spectroscopy
	Ma HongFei ; Zhang Yong ; Ye AnPei
刊名	科学通报英文版
	2013
关键词	Raman spectroscopy optical tweezers human erythrocyte single cell identification principle components analysis AQUEOUS-SOLUTION IDENTIFICATION STATE
DOI	10.1007/s11434-013-5721-6
英文摘要	The ability to discriminate between single cells in a label-free and noninvasive fashion is important for the classification of cells, and for the identification of similar cells from different origins. In this paper, we present the Raman spectroscopy-based identification of different types of single cells in aqueous media, and discrimination between the same types of cells from different donors using a novel Laser Tweezers Raman Spectroscopy (LTRS) technique, which combines laser trapping and micro-Raman spectroscopy. First, we measured the spectra of individual living human erythrocytes, i.e. red blood cells, and leucocytes (U937 cancer cells). High-quality Raman spectra with low fluorescence were obtained using a home-LTRS apparatus and 20 cells were measured for each cell type. The smoothing, baseline subtraction, and normalization of the data were followed by a principal components analysis (PCA). The PCA loading plots showed that the two different types of cells could be completely separated based only on the first component (PC1) (i.e. the peaks at 1300 cm(-1)); the discrimination accuracy could therefore reach 100%. More than 50 spectra were taken for each erythrocyte obtained from the four healthy volunteers. The average discrimination accuracy was 84.5% for two random individuals taken from the four volunteers, according to the first and second PCs. This work demonstrates that LTRS is a powerful tool for the accurate identification and discrimination of single cells, and it has the potential to be applied for the highly sensitive identification of cells in clinical diagnosis and medical jurisprudence.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000322035700010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 1; ARTICLE; 21,SI; 2594-2600; 58
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/152287]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Ma HongFei,Zhang Yong,Ye AnPei. Single-cell discrimination based on optical tweezers Raman spectroscopy[J]. 科学通报英文版,2013.
APA	Ma HongFei,Zhang Yong,&Ye AnPei.(2013).Single-cell discrimination based on optical tweezers Raman spectroscopy.科学通报英文版.
MLA	Ma HongFei,et al."Single-cell discrimination based on optical tweezers Raman spectroscopy".科学通报英文版 (2013).