Ammonia manipulates the ammonia-oxidizing archaea and bacteria in the   coastal sediment-water microcosms

doi:10.1007/s00253-015-6524-2

CORC > 北京大学 > 环境科学与工程学院

	Ammonia manipulates the ammonia-oxidizing archaea and bacteria in the coastal sediment-water microcosms
	Zhang, Yan ; Chen, Lujun ; Dai, Tianjiao ; Sun, Renhua ; Wen, Donghui
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2015
关键词	Ammonia-oxidizing archaea Ammonia-oxidizing bacteria Sediment microcosm Stable isotope probing Transcriptional activity AGRICULTURAL SOIL NITRIFYING ARCHAEA OXIDATION DIVERSITY ABUNDANCE NITRIFICATION MICROORGANISMS THAUMARCHAEA COMMUNITIES CULTIVATION
DOI	10.1007/s00253-015-6524-2
英文摘要	Ammonia was observed as a potential significant factor to manipulate the abundance and activity of ammonia-oxidizing microorganisms (AOMs) in water environments. For the first time, this study confirmed this phenomenon by laboratory cultivation. In a series of estuarine sediment-coastal water microcosms, we investigated the AOM's phylogenetic composition and activity change in response to ammonia concentration. Increase of ammonia concentration promoted bacterial amoA gene abundance in a linear pattern. The ratio of transcribed ammonia-oxidizing bacteria (AOB) amoA gene/ammonia-oxidizing archaea (AOA) amoA gene increased from 0.1 to 43 as NH4 (+)-N increased from less than 0.1 to 12 mg L-1, and AOA amoA transcription was undetected under 20 mg NH4 (+)-N L-1. The incubation of stable isotope probing (SIP) microcosms revealed a faster C-13-NaHCO3 incorporation rate of AOA amoA gene under 0.1 mg NH4 (+)-N L-1 and a sole C-13-NaHCO3 utilization of the AOB amoA gene under 20 mg NH4 (+)-N L-1. Our results indicate that ammonia concentration manipulates the structure of AOM. AOA prefers to live and perform higher amoA transcription activity than AOB in ammonia-limited water environments, and AOB tends to take the first contributor place in ammonia-rich ones.; Natural Science Foundation of China [51178002]; SCI(E); EI; ARTICLE; dhwen@pku.edu.cn; 15; 6481-6491; 99
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/417893]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Zhang, Yan,Chen, Lujun,Dai, Tianjiao,et al. Ammonia manipulates the ammonia-oxidizing archaea and bacteria in the coastal sediment-water microcosms[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2015.
APA	Zhang, Yan,Chen, Lujun,Dai, Tianjiao,Sun, Renhua,&Wen, Donghui.(2015).Ammonia manipulates the ammonia-oxidizing archaea and bacteria in the coastal sediment-water microcosms.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY.
MLA	Zhang, Yan,et al."Ammonia manipulates the ammonia-oxidizing archaea and bacteria in the coastal sediment-water microcosms".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2015).