不动杆菌(Acinetobacter sp.)降解4-氯酚的特性及机制研究

CORC > 北京大学 > 环境科学与工程学院

	不动杆菌(Acinetobacter sp.)降解4-氯酚的特性及机制研究; Characteristics of 4-chlorophenol degradation by a soil bacterium Acinetobacter sp
	吴为中 ; 冯叶成 ; 王建龙
刊名	环境科学
	2008
关键词	氯酚生物降解优先污染物酶活性裂解途径
英文摘要	富集培养从受污染土壤中分离到的能够以4-氯酚为唯一碳源和能源的微生物,16S rDNA序列分析表明,该微生物为Acinetobacter sp..其降解4-氯酚的机制为邻位裂解途径,氯代邻苯二酚1,2-双加氧酶的活性可以通过氯酚的诱导显著提高.当氯酚的初始浓度范围为2~8 mmol/L时,该微生物能够很好地生长,并能有效地降解氯酚.除4-氯酚外,该微生物还可以降解2-氯酚、3-氯酚和2,4-二氯酚,有较宽的底物范围.添加柠檬酸等共基质不仅能够改善微生物的生长,还可以提高氯酚的降解效率,这对于实际受污染环境的生物修复非常重要.; The bacterium capable of degrading 4-chlorophenol (4-CP) was enriched and isolated from agricultural soil. It was gram-negative, rod-shaped bacterium, and identified as Acinetobacter sp. based on the analysis of the 16S rRNA gene fragment. It was able to utilize 4-CP as a sole carbon source. The degradation mechanism of 4-CP by this isolate was proposed as a modified ortho-cleavage pathway, the activity of chlorocatechol 1,2-dioxygenase was markedly induced. The bacterial isolate grew well and exhibited a high degradation efficiency when the initial concentration of 4-CP was between 2-8 mmol/L. It was able to survive in the presence of 4-CP at higher concentrations (up to 8 mmol/L). Not only 4-CP, but also 2-chlorophenol, 3-chlorophenol, phenol, and 2,4-dichlorophenol, were also growth substrates for the isolate. The results of co-substrate supplementation illustrated the suitable conditions of the isolate to improve growth rate and 4-chlorophenol biodegradation efficiency. The results suggested that the isolate had a potential use for bioremediation of the site contaminated with 4-chlorophenol.; PubMed; 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 0; 11; 3185-3188; 29
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/324339]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	吴为中,冯叶成,王建龙. 不动杆菌(Acinetobacter sp.)降解4-氯酚的特性及机制研究, Characteristics of 4-chlorophenol degradation by a soil bacterium Acinetobacter sp[J]. 环境科学,2008.
APA	吴为中,冯叶成,&王建龙.(2008).不动杆菌(Acinetobacter sp.)降解4-氯酚的特性及机制研究.环境科学.
MLA	吴为中,et al."不动杆菌(Acinetobacter sp.)降解4-氯酚的特性及机制研究".环境科学 (2008).