Identification of ubiquinones and menaquinones in activated sludge by   liquid chromatography-atmospheric pressure chemical ionization mass   spectrometry

doi:10.1016/S0021-9673(03)00930-0

CORC > 北京大学 > 环境科学与工程学院

	Identification of ubiquinones and menaquinones in activated sludge by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry
	Gao, MC ; Yang, M ; Hu, HY ; Shao, B ; Zhang, HF ; Li, HY
刊名	journal of chromatography a
	2003
关键词	activated sludge environmental analysis ubiquinones menaquinones RESPIRATORY QUINONE PROFILES WASTE-WATER TREATMENT BACTERIA MIXTURES
DOI	10.1016/S0021-9673(03)00930-0
英文摘要	A sensitive analytical method has been developed for identification of ubiquinones (UQ-n(H-x)) and menaquinones (MK-n(H-x)) in activated sludge by liquid chromatography - atmospheric pressure chemical ionization mass spectrometry in negative mode (LC-NI-APCI-MS). Extraction and clean-up of samples were carried out on Sep-Pak Plus Silica solid-phase extraction cartridges. Complete separation of quinones was achieved with an ODS analytical column and using isopropyl ether-methanol (17:83, v/v) as the mobile phase. The compositions of ubiquinones and menaquinones were determined directly using combined information on retention time, the molecular ion mass and fragment ion masses. The lowest instrument quantitative detection limits (LODinst) for UQ-6, UQ-10, and Vitamin K1 were estimated to be 0.4, 4 and 0.12 ng (S/N = 10) using LC-NI-APCI-MS in SIM mode, and the lowest method detection limits (LODmeth) achieved by spiking experiment were estimated to be 0.2, 2 and 0.06 mug/g for UQ-6, UQ-10 and Vitamin K1, respectively. On the other hand, the LODinst for UQ-6, UQ-10, and Vitamin K1 were estimated to be 10, 100 and 2 ng (S/N= 10) using LC-NI-APCI-MS in full-scan mode, and the LODmeth were estimated to be 7, 60 and 1.2 mug/g for UQ-6, UQ-10, and Vitamin K1, respectively. Both LC-NI-APCI-MS and LC-UV/DAD were applied in the analysis of an activated sludge extract. UQ-n (n=6-10), MK-n (n=6-10), MK-n(H-2) (n=7-10), MK-n(H-4) (n-8-9) and MK-8(H-6) were detected by LC-NI-APCI-MS, while UQ-6. UQ-7, MK-7(H-2). MK-9 and MK-10(H-2) were not found by LC-UV/DAD. These results suggest that LC-NI-APCI-MS is more sensitive than LC-UV/DAD for the analysis of quinones in environmental samples such as sediment, activated sludge and bio-film in biological processes and other aquatic environments. (C) 2003 Elsevier B.V. All rights reserved.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); EI; 0; ARTICLE; 1-2; 31-37; 1007
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/256449]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Gao, MC,Yang, M,Hu, HY,et al. Identification of ubiquinones and menaquinones in activated sludge by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry[J]. journal of chromatography a,2003.
APA	Gao, MC,Yang, M,Hu, HY,Shao, B,Zhang, HF,&Li, HY.(2003).Identification of ubiquinones and menaquinones in activated sludge by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.journal of chromatography a.
MLA	Gao, MC,et al."Identification of ubiquinones and menaquinones in activated sludge by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry".journal of chromatography a (2003).