Induction of vitellogenin mRNA in juvenile Chinese sturgeon (Acipenser   sinensis Gray) treated with 17 beta-estradiol and 4-nonylphenol

doi:10.1897/04-436R.1

CORC > 北京大学 > 环境科学与工程学院

	Induction of vitellogenin mRNA in juvenile Chinese sturgeon (Acipenser sinensis Gray) treated with 17 beta-estradiol and 4-nonylphenol
	Zhang, ZB ; Hu, JY ; An, W ; Jin, F ; An, LH ; Tao, S ; Chen, JS
刊名	environmental toxicology and chemistry
	2005
关键词	endocrine-disrupting chemicals real-time polymerase chain reaction vitellogenin 4-nonylphenol Acipenser sinensis MEDAKA ORYZIAS-LATIPES P-NONYLPHENOL ZONA-RADIATA EXPRESSION TROUT CHEMICALS FISH GROWTH RIVER PCR
DOI	10.1897/04-436R.1
英文摘要	It has been demonstrated that 4-nonylphenol (4-NP) exerts estrogenic effects in diverse fishes. The present study investigated the effects of 4-NP on Chinese sturgeon (Acipenser sinensis Gray) vitellogenin (VTG) gene expression. By reverse transcription-polymerase chain reaction (RT-PCR) using degenerate primers, a 462-base pair fragment of Chinese sturgeon VTG, corresponding to a 154-amino acid sequence, was amplified and sequenced. This sequence exhibited 152/154 identity to the amino acid sequence of white sturgeon (A. transmontano) VTG. Conventional RT-PCR and quantitative real-time RT-PCR were established and used to study the VTG and P-actin gene expression in the liver of juvenile Chinese sturgeon injected three times with 17 beta-estradiol (5 mg/kg body wt/week) or 4-NP (10 or 100 mg/kg/week) in the course of three weeks. Significant induction of VTG gene expression was detected in all treated groups, and no VTG mRNA was detected in the control group. The ratio of VTG to beta-actin analyzed from the results of quantitative real-time RT-PCR reached 0.041 +/- 0.024 (mean SD) in the group receiving 10 mg/kg/week of 4-NP and 4.51 +/- 1.68 in the group receiving 100 mg/kg/week of 4-NP. Chemical analysis of 4-NP showed that the concentrations of 4-NP in the 10 mg/kg/week group and the 100 mg/kg/week group were 2.78 +/- 2.41 and 31.38 +/- 0.26 mu g/g wet weight, respectively. Compared with the 4-NP concentrations (0.8-1.92 mu g/g wet wt) in fish from the Yangtze River, China, a potential hazard exists regarding 4-NP in Chinese sturgeon. These results represent the first indication of the risk of endocrinedisrupting chemicals for Chinese sturgeon.; Environmental Sciences; Toxicology; SCI(E); EI; 16; 8; 1944-1950; 24
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/253148]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Zhang, ZB,Hu, JY,An, W,et al. Induction of vitellogenin mRNA in juvenile Chinese sturgeon (Acipenser sinensis Gray) treated with 17 beta-estradiol and 4-nonylphenol[J]. environmental toxicology and chemistry,2005.
APA	Zhang, ZB.,Hu, JY.,An, W.,Jin, F.,An, LH.,...&Chen, JS.(2005).Induction of vitellogenin mRNA in juvenile Chinese sturgeon (Acipenser sinensis Gray) treated with 17 beta-estradiol and 4-nonylphenol.environmental toxicology and chemistry.
MLA	Zhang, ZB,et al."Induction of vitellogenin mRNA in juvenile Chinese sturgeon (Acipenser sinensis Gray) treated with 17 beta-estradiol and 4-nonylphenol".environmental toxicology and chemistry (2005).