A comparison of the efficiencies of a portable BioStage impactor and a   Reuter centrifugal sampler (RCS) High Flow for measuring airborne   bacteria and fungi concentrations

doi:10.1016/j.jaerosci.2009.02.003

CORC > 北京大学 > 环境科学与工程学院

	A comparison of the efficiencies of a portable BioStage impactor and a Reuter centrifugal sampler (RCS) High Flow for measuring airborne bacteria and fungi concentrations
	Zhen, Shiqi ; Li, Kejun ; Yin, Lihong ; Yao, Maosheng ; Zhang, Huili ; Chen, Liansheng ; Zhou, Minghao ; Chen, Xiaodong
刊名	journal of aerosol science
	2009
关键词	Portable BioStage impactor Reuter centrifugal sampler (RCS) High Flow Efficiencies Bioaerosol qPCR REAL-TIME PCR MICROBIAL SAMPLERS AIR SAMPLERS COLLECTION MICROORGANISMS QUANTIFICATION BIOAEROSOLS ENUMERATION EXPOSURE DESIGN
DOI	10.1016/j.jaerosci.2009.02.003
英文摘要	The biological collection efficiencies of the portable BioStage impactor and the Reuter centrifugal sampler (RCS) High Flow were investigated when measuring bacteria and fungi concentrations in five different environments in Shanghai, China. The devices were operated simultaneously at flow rates of 28.3 and 100 L/min, respectively, in the same environment for 5 min. Air samples were taken three times (morning, afternoon and evening) for culturable microorganisms and the entire experiments were repeated three times. In addition, the efficiencies of collecting the total bacteria by each of the samplers using different sampling time were quantified by quantitative polymerase chain reaction (qPCR). Gel electrophoresis was used to analyze the resulting qPCR products. The results indicate that, in general, the portable BioStage impactor yielded higher culturable bacterial and fungal concentrations than the RCS High Flow. Paired t-tests indicate that the differences were statistically significant in most cases. The qPCR tests show that the efficiencies of the two samplers depend on the sampling time and the environment. Among other factors, the particle bounce, the sampling time, and the desiccation play major roles in the observed sampling differences between the samplers. (C) 2009 Elsevier Ltd. All rights reserved.; Engineering, Chemical; Engineering, Mechanical; Environmental Sciences; Meteorology & Atmospheric Sciences; SCI(E); EI; 16; ARTICLE; 6; 503-513; 40
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/245765]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Zhen, Shiqi,Li, Kejun,Yin, Lihong,et al. A comparison of the efficiencies of a portable BioStage impactor and a Reuter centrifugal sampler (RCS) High Flow for measuring airborne bacteria and fungi concentrations[J]. journal of aerosol science,2009.
APA	Zhen, Shiqi.,Li, Kejun.,Yin, Lihong.,Yao, Maosheng.,Zhang, Huili.,...&Chen, Xiaodong.(2009).A comparison of the efficiencies of a portable BioStage impactor and a Reuter centrifugal sampler (RCS) High Flow for measuring airborne bacteria and fungi concentrations.journal of aerosol science.
MLA	Zhen, Shiqi,et al."A comparison of the efficiencies of a portable BioStage impactor and a Reuter centrifugal sampler (RCS) High Flow for measuring airborne bacteria and fungi concentrations".journal of aerosol science (2009).