A systematic insight into a single-stage deammonification process   operated in granular sludge reactor with high-loaded reject-water:   characterization and quantification of microbiological community

doi:10.1111/jam.12042

CORC > 北京大学 > 环境科学与工程学院

	A systematic insight into a single-stage deammonification process operated in granular sludge reactor with high-loaded reject-water: characterization and quantification of microbiological community
	Liu, S. T. ; Horn, H. ; Mueller, E.
刊名	journal of applied microbiology
	2013
关键词	anammox bacteria cloning deammonification FISH PCR-DGGE reject-water ANAEROBIC AMMONIUM OXIDATION 16S RIBOSOMAL-RNA IN-SITU DETECTION AUTOTROPHIC NITROGEN REMOVAL INDUSTRIAL WASTE-WATER ACTIVATED-SLUDGE ANAMMOX BACTERIA PARTIAL NITRITATION OLIGONUCLEOTIDE PROBES MICROBIAL-POPULATIONS
DOI	10.1111/jam.12042
英文摘要	Aims Reject-water from sludge dewatering was treated in a single-stage deammonification reactor. The aims were to characterize the microbiological community within deammonification granules. Methods and Results In situ mapping of the intact granular sludge were made with fluorescent in situ hybridization (FISH). The Planctomycetes community in the destroyed granular sludge was characterized by FISH, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), cloning and sequence analysis. Conclusions AOB within Betaproteobacteria were concentrated within the first 50100 mu m of the granule, and Planctomycetes in the first 100200 mu m were responsible for nitrogen elimination. PCR-DGGE verified the higher diversity of Planctomycetes in the deammonification reactor than the anaerobic cultivation. The sequence analysis after PCR-DGGE and cloning identified the dominant Planctomycetes species for anammox reaction as Ca. Brocadia fulgida (accession no. EU478693). FISH detection using the universal probe AMX368 specific for all anammox bacteria including Ca. Brocadia fulgida failed; however, the probe BFU613 specific for Ca. Brocadia fulgida gave clear positive FISH signals. The three-dimensional structure of the ribosome may hinder binding of the universal probe to the corresponding 16S rRNA region. Significance and Impact of the Study A combination of multiple methods for the analysis of the microbiological community was necessary. Oligonucleotide probes should be carefully selected, and a negative FISH analysis has to be verified by other molecular biological techniques.; Biotechnology & Applied Microbiology; Microbiology; SCI(E); 0; ARTICLE; 2; 339-351; 114
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/239888]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Liu, S. T.,Horn, H.,Mueller, E.. A systematic insight into a single-stage deammonification process operated in granular sludge reactor with high-loaded reject-water: characterization and quantification of microbiological community[J]. journal of applied microbiology,2013.
APA	Liu, S. T.,Horn, H.,&Mueller, E..(2013).A systematic insight into a single-stage deammonification process operated in granular sludge reactor with high-loaded reject-water: characterization and quantification of microbiological community.journal of applied microbiology.
MLA	Liu, S. T.,et al."A systematic insight into a single-stage deammonification process operated in granular sludge reactor with high-loaded reject-water: characterization and quantification of microbiological community".journal of applied microbiology (2013).