喹啉降解菌Rhodococcus sp.QL2的分离鉴定及降解特性

doi:10.3321/j.issn:0250-3301.2008.02.035

CORC > 北京大学 > 环境科学与工程学院

	喹啉降解菌Rhodococcus sp.QL2的分离鉴定及降解特性; Isolation, Identification and Degradation Characteristics of a Quinoline-Degrading Bacterium Rhodococcus sp. QL2
	朱顺妮 ; 刘冬启 ; 樊丽 ; 倪晋仁
刊名	环境科学
	2008
关键词	Rhodococcus sp. 分离鉴定系统发育降解特性动力学
DOI	10.3321/j.issn:0250-3301.2008.02.035
英文摘要	从某焦化厂生物处理系统的活性污泥中驯化、分离出1株能以喹啉为唯一碳、氮、能源生长代谢的菌株QL2.经过对其形态特征、生理生化特征和16S rRNA序列分析鉴定该菌株为红球菌属(Rhodococcus sp.).研究表明,菌株QL2利用喹啉生长的适宜温度为35～42℃,培养基初始pH为8～9,摇床转速为150 r/min.外加氮源能促进菌株的生长,其中无机氮比有机氮、铵态氮比硝态氮更利于细菌的生长.在喹啉初始浓度为60～680 mg/L范围内菌株QL2降解喹啉符合零级动力学方程.喹啉初始浓度为150 mg/L时在8 h内完全降解,TOC去除率14 h内可达到70%.降解过程中产生有颜色的物质,且杂环上的氮原子以氨氮的形式被释放.通过HPLC及GC/MS分析出喹啉降解过程中的主要中间产物为2-羟基喹啉.该菌底物利用范围广,能降解苯酚、萘、吡啶等多种芳香族化合物.; A quinoline-degrading bacterium QL2, which utilizes quinoline as sole source of carbon, nitrogen and energy, was isolated from activated sludge in a coke-plant wastewater biological treatment system. According to the morphological characteristics, physiological and biochemical characteristics, and sequence analysis of 16S rRNA, the strain was identified as Rhodococcus sp.. The optimal temperature, initial pH, and shaker rotary speed for strain QL2 utilizing quinoline are 35-42 degrees C, pH 8-9, and 150 r/min, respectively. Extra nitrogen sources stimulate the isolate growth on quinoline, and inorganic nitrogen better than organic nitrogen, NH4+ -N better than NO3(-) -N. The degradation reaction of quinoline by strain QL2 can be described with zero order kinetic equation within the initial quinoline concentrations of 60-680 mg/L. When the initial concentration was 150 mg/L, quinoline was degraded completely in 8 hours and TOC removal efficiency was 70% in 14 hours. This bacterium produced pigmented compounds, and ring nitrogen was released into the growth medium as ammonium. The main intermediate in the degradation pathway was 2-hydroxyquinoline by the analysis of HPLC and GC/MS. With a broad range of substrate utilization, the strain can degrade phenol, naphthalene, pyridine, and some other kinds of aromatic compounds.; 国家自然科学基金; PubMed; 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 0; 2; 488-493; 29
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/207703]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	朱顺妮,刘冬启,樊丽,等. 喹啉降解菌Rhodococcus sp.QL2的分离鉴定及降解特性, Isolation, Identification and Degradation Characteristics of a Quinoline-Degrading Bacterium Rhodococcus sp. QL2[J]. 环境科学,2008.
APA	朱顺妮,刘冬启,樊丽,&倪晋仁.(2008).喹啉降解菌Rhodococcus sp.QL2的分离鉴定及降解特性.环境科学.
MLA	朱顺妮,et al."喹啉降解菌Rhodococcus sp.QL2的分离鉴定及降解特性".环境科学 (2008).