Identification of a novel gentisate 1,2-dioxygenase from Silicibacter   pomeroyi

doi:10.1007/s10529-007-9421-7

CORC > 北京大学 > 环境科学与工程学院

	Identification of a novel gentisate 1,2-dioxygenase from Silicibacter pomeroyi
	Liu, Dongqi ; Zhu, Tingting ; Fan, Li ; Quan, Junming ; Guo, Hongchun ; Ni, Jinren
刊名	biotechnology letters
	2007
关键词	Gentisate 1 2-dioxygenase purification recombinant Silicibacter pomeroyi KLEBSIELLA-PNEUMONIAE M5A1 BIOCHEMICAL-CHARACTERIZATION PSEUDOMONAS-TESTOSTERONI SEQUENCE ALIGNMENT PURIFICATION GENES ACIDOVORANS SOFTWARE ENZYMES PATHWAY
DOI	10.1007/s10529-007-9421-7
英文摘要	A 1,125-bp long ORF encoding a novel gentisate 1,2-dioxygenase with two-domain bicupins was cloned from Silicibacter pomeroyi DSS-3 and expressed in Escherichia coli. The resulting product was purified to homogeneity and partially characterized. Non-reductive SDS-PAGE and gel filtration showed that the active recombinant gentisate 1,2-dioxygenase had an estimated molecular mass of 132 kDa, and reductive SDS-PAGE indicated an approximate size of 45 kDa. The enzyme thus appears to be a homotrimeric protein. This is in contrast to the homotetrameric or dimeric protein of the gentisate 1,2-dioxygenases that have been characterized thus far. The K-m and K-cat/K-m for gentisate were 12 mu M and 653 x 10(4) M-1 s(-1); the pI was 4.6-4.8. It was optimally active at 40 degrees C and pH 8.0.; Biotechnology & Applied Microbiology; SCI(E); EI; PubMed; 2; ARTICLE; 10; 1529-1535; 29
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/158983]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Liu, Dongqi,Zhu, Tingting,Fan, Li,et al. Identification of a novel gentisate 1,2-dioxygenase from Silicibacter pomeroyi[J]. biotechnology letters,2007.
APA	Liu, Dongqi,Zhu, Tingting,Fan, Li,Quan, Junming,Guo, Hongchun,&Ni, Jinren.(2007).Identification of a novel gentisate 1,2-dioxygenase from Silicibacter pomeroyi.biotechnology letters.
MLA	Liu, Dongqi,et al."Identification of a novel gentisate 1,2-dioxygenase from Silicibacter pomeroyi".biotechnology letters (2007).