Influence of solution chemistry on the deposition and detachment   kinetics of RNA on silica surfaces

doi:10.1016/j.colsurfb.2010.09.018

CORC > 北京大学 > 环境科学与工程学院

	Influence of solution chemistry on the deposition and detachment kinetics of RNA on silica surfaces
	Shen, Yun ; Kim, Hyunjung ; Tong, Meiping ; Li, Qingyun
刊名	colloids and surfaces b biointerfaces
	2011
关键词	RNA Deposition kinetics Detachment DLVO QCM-D QUARTZ-CRYSTAL MICROBALANCE NATURAL ORGANIC-MATTER PLASMID DNA GENETIC MATERIAL DIVALENT SALTS ADSORPTION ACID PROTEIN HYBRIDIZATION BACTERIOPHAGE-MS2
DOI	10.1016/j.colsurfb.2010.09.018
英文摘要	The deposition kinetics of RNA extracted from both virus and bacteria on silica surfaces were examined in both monovalent (NaCl) and divalent (CaCl2) solutions under a wide range of environmentally relevant ionic strength and pH conditions by utilizing a quartz crystal microbalance with dissipation (QCM-D). To better understand the RNA deposition mechanisms, QCM-D data were complemented by diffusion coefficients and zeta potentials of RNA as a function of examined solution chemistry conditions. Favorable deposition of RNA on poly-L-lysine-coated (positively charged) silica surfaces was governed by the convective-diffusive transport of RNA to the surfaces. The deposition kinetics of RNA on bare silica surfaces were controlled by classic Derjaguin-Landau-Verwey-Overbeek (DLVO) interactions. The presence of divalent cations (Ca2+) in solutions greatly enhanced the deposition kinetics of RNA on silica surfaces. Solution pH also affected the deposition behavior of RNA on silica surfaces. Release experiments showed that detachment of RNA from silica surfaces was significant in NaCl solutions, whereas, the deposited RNA on silica surfaces in CaCl2 solutions was more likely to be irreversible. (C) 2010 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000285858200027&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biophysics; Chemistry, Physical; Materials Science, Biomaterials; SCI(E); EI; PubMed; 4; ARTICLE; 2; 443-449; 82
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/158898]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Shen, Yun,Kim, Hyunjung,Tong, Meiping,et al. Influence of solution chemistry on the deposition and detachment kinetics of RNA on silica surfaces[J]. colloids and surfaces b biointerfaces,2011.
APA	Shen, Yun,Kim, Hyunjung,Tong, Meiping,&Li, Qingyun.(2011).Influence of solution chemistry on the deposition and detachment kinetics of RNA on silica surfaces.colloids and surfaces b biointerfaces.
MLA	Shen, Yun,et al."Influence of solution chemistry on the deposition and detachment kinetics of RNA on silica surfaces".colloids and surfaces b biointerfaces (2011).