Integration of high volume portable aerosol-to-hydrosol sampling and   qPCR in monitoring bioaerosols

doi:10.1039/c0em00559b

CORC > 北京大学 > 环境科学与工程学院

	Integration of high volume portable aerosol-to-hydrosol sampling and qPCR in monitoring bioaerosols
	He, Qishuang ; Yao, Maosheng
刊名	环境监测杂志
	2011
关键词	REAL-TIME PCR AIRBORNE MICROORGANISMS MICROBIAL SAMPLERS AIR SAMPLERS COLLECTION QUANTIFICATION PERFORMANCE ENUMERATION EXPOSURE
DOI	10.1039/c0em00559b
英文摘要	In this study, the integration of a high volume, portable aerosol-to-hydrosol sampling technique and quantitative polymerase chain reaction (qPCR) was investigated for bioaerosol monitoring by adapting the RCS High Flow to sample air with mineral-oil-strips. Bacillus subtilis var niger and Pseudomonas fluorescens were aerosolized and collected by the RCS High Flow loaded with mineral-oil-strips for 1, 2 and 5 min. In addition, the adapted aerosol-to-hydrosol sampler was also tested for sampling environmental bacterial aerosols in four different environments (a back yard, a student dorm, a dining hall, and a play ground). The performances of the RCS High Flow with mineral-oil-strips were compared with the use of agar strips under similar conditions in all experiments. Air samples collected by the RCS High Flow were cultured, and in addition those collected with mineral-oil-strips were also quantified using qPCR. When sampling B. subtilis var niger aerosols, the use of mineral-oil-strips was shown to report significantly higher culturable concentrations than those obtained by agar strips regardless of the sampling time tested (p-value = 0.04). In contrast, the differences between the two methods when sampling P. fluorescens aerosols were not statistically significant (p-value = 0.5). When coupled with qPCR, the RCS High Flow loaded with mineral-oil-strips obtained significantly higher bacterial aerosol concentrations than those detected by the culturing method. The sampling time was observed to have negligible effects on the efficiency of the technology developed here. When sampling in different environments, the use of mineral-oil-strip was observed to yield significantly higher, about 4-12 times, culturable bacterial aerosol concentration levels compared to the use of agar. This study demonstrated a high volume (100 L min(-1)) portable aerosol-to-hydrosol sampling technique, holding broad promise in monitoring airborne biological threats when coupled with qPCR technology. Yet, caution should be taken in relating the bioaerosol concentrations to health risks as qPCR detects both culturable and non-culturable cells including inactivated ones.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000288160200026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; Environmental Sciences; SCI(E); PubMed; 7; ARTICLE; 3; 706-712; 13
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/158881]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	He, Qishuang,Yao, Maosheng. Integration of high volume portable aerosol-to-hydrosol sampling and qPCR in monitoring bioaerosols[J]. 环境监测杂志,2011.
APA	He, Qishuang,&Yao, Maosheng.(2011).Integration of high volume portable aerosol-to-hydrosol sampling and qPCR in monitoring bioaerosols.环境监测杂志.
MLA	He, Qishuang,et al."Integration of high volume portable aerosol-to-hydrosol sampling and qPCR in monitoring bioaerosols".环境监测杂志 (2011).