Production of diosgenin from Dioscorea zingiberensis tubers through   enzymatic saccharification and microbial transformation

doi:10.1007/s00253-009-2200-8

CORC > 北京大学 > 环境科学与工程学院

	Production of diosgenin from Dioscorea zingiberensis tubers through enzymatic saccharification and microbial transformation
	Zhu, Yu-Ling ; Huang, Wen ; Ni, Jin-Ren ; Liu, Wei ; Li, Hui
刊名	applied microbiology and biotechnology
	2010
关键词	Diosgenin yield Enzymatic saccharification Microbial transformation Saponins Trichoderma reesei CURVULARIA-LUNATA CELLS BIOTRANSFORMATION HYDROLYSIS CONVERSION APOPTOSIS SAPONINS
DOI	10.1007/s00253-009-2200-8
英文摘要	In order to develop a clean and effective approach for producing the valuable drug diosgenin from Dioscorea zingiberensis tubers, two successive processes, enzymatic saccharification and microbial transformation, were used. With enzymatic saccharification, 98.0% of starch was excluded from the raw herb, releasing saponins from the network structure of starch. Subsequently, the treated tubers were fermented with Trichoderma reesei under optimal conditions for 156 h. During microbial transformation, glycosidic bonds, which link beta-d-glucose or alpha-l-rhamnose with aglycone at the C-3 position in saponins, were broken down effectively to give a diosgenin yield of 90.6 +/- 2.45%, 42.4% higher than that obtained from bioconversion of raw tubers directly. Scaled up fermentation was conducted in a 5.0-l bioreactor and gave a diosgenin yield of 91.2 +/- 3.21%. This is the first report on the preparation of diosgenin from herbs through microbial transformation as well as utilizing other available components in the raw material, providing an environmentally friendly alternative to diosgenin production.; Biotechnology & Applied Microbiology; SCI(E); EI; 10; ARTICLE; 5; 1409-1416; 85
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/158514]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Zhu, Yu-Ling,Huang, Wen,Ni, Jin-Ren,et al. Production of diosgenin from Dioscorea zingiberensis tubers through enzymatic saccharification and microbial transformation[J]. applied microbiology and biotechnology,2010.
APA	Zhu, Yu-Ling,Huang, Wen,Ni, Jin-Ren,Liu, Wei,&Li, Hui.(2010).Production of diosgenin from Dioscorea zingiberensis tubers through enzymatic saccharification and microbial transformation.applied microbiology and biotechnology.
MLA	Zhu, Yu-Ling,et al."Production of diosgenin from Dioscorea zingiberensis tubers through enzymatic saccharification and microbial transformation".applied microbiology and biotechnology (2010).