Process optimization for the production of diosgenin with Trichoderma   reesei

doi:10.1007/s00449-009-0390-1

CORC > 北京大学 > 环境科学与工程学院

	Process optimization for the production of diosgenin with Trichoderma reesei
	Zhu, Yuling ; Ni, Jinren ; Huang, Wen
刊名	bioprocess and biosystems engineering
	2010
关键词	Diosgenin yield Saponins Trichoderma reesei Central composite design Microbial transformation RESPONSE-SURFACE METHODOLOGY ZINGIBERENSIS HYDROLYSIS ENZYMES CELLULASE CULTURES GROWTH
DOI	10.1007/s00449-009-0390-1
英文摘要	Based on the response surface methodology, an effective microbial system for diosgenin production from enzymatic pretreated Dioscorea zingiberensis tubers with Trichoderma reesei was studied. The fermentation medium was optimized with central composite design (3(5)) depended on Plackett-Burmann design which identified significant impacts of peptone, K(2)HPO(4) and Tween 80 on diosgenin yield. The effects of different fermentation conditions on diosgenin production were also studied. Four parameters, i.e. incubation period, temperature, initial pH and substrate concentration were optimized using 4(5) central composite design. The highest diosgenin yield of 90.57% was achieved with 2.67% (w/v) of peptone, 0.29% (w/v) of K(2)HPO(4), 0.73% (w/v) of Tween 80 and 9.77% (w/v) of substrate, under the condition of pH 5.8, temperature 30 A degrees C. The idealized incubation time was 6.5 days. After optimization, the product yield increased by 33.70% as compared to 67.74 +/- A 1.54% of diosgenin yield in not optimized condition. Scale-up fermentation was carried out in a 5.0 l bioreactor, maximum diosgenin yield of 90.17 +/- A 3.12% was obtained at an aeration of 0.80 vvm and an agitation rate of 300 rpm. The proposed microbial system is clean and effective for diosgenin production and thus more environmentally acceptable than the traditional acid hydrolysis.; Biotechnology & Applied Microbiology; Engineering, Chemical; SCI(E); EI; 9; ARTICLE; 5; 647-655; 33
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/158484]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Zhu, Yuling,Ni, Jinren,Huang, Wen. Process optimization for the production of diosgenin with Trichoderma reesei[J]. bioprocess and biosystems engineering,2010.
APA	Zhu, Yuling,Ni, Jinren,&Huang, Wen.(2010).Process optimization for the production of diosgenin with Trichoderma reesei.bioprocess and biosystems engineering.
MLA	Zhu, Yuling,et al."Process optimization for the production of diosgenin with Trichoderma reesei".bioprocess and biosystems engineering (2010).