Comparison of the biological content of air samples collected at ground   level and at higher elevation

doi:10.1007/s10453-010-9159-x

CORC > 北京大学 > 环境科学与工程学院

	Comparison of the biological content of air samples collected at ground level and at higher elevation
	Li, Kejun ; Dong, Shuofei ; Wu, Yan ; Yao, Maosheng
刊名	aerobiologia
	2010
关键词	Culturability DGGE DNA sequencing qPCR Bacterial diversity Elevation Fungi RIBOSOMAL-RNA SOLAR-RADIATION AIRBORNE FUNGI METEOROLOGICAL FACTORS HOME CHARACTERISTICS EXPOSURE ASSESSMENT PARTICULATE MATTER MOUTH-DISEASE DESERT DUST INDOOR
DOI	10.1007/s10453-010-9159-x
英文摘要	In this study, air samples were collected simultaneously at ground level and from a broadcast tower (open space) 238 m high using two BioSamplers and two portable Biostage impactors. The sampling lasted up to 5 h, from morning to afternoon, and the experiments were independently conducted twice. The samples collected by the BioSamplers were analyzed for bacterial diversity and concentration, endotoxin and (1,3)-beta-d-glucan content. Denaturing gradient gel electrophoresis (DGGE) fingerprinting, DNA sequencing, real-time quantitative polymerase chain reaction (qPCR), and Limulus amebocyte lysate (LAL) were used for the analyses. Air samples collected by the portable Biostage impactors were directly incubated at 25A degrees C, and the culturable bacteria and fungi concentrations were manually counted. The results from the culture analysis indicated that the ground air samples had higher (statistically significant) concentrations of culturable bacteria and fungi than those taken at an elevation of 238 m. DGGE profiles of PCR products showed similar bacterial diversity in both ground-level and elevated air samples. Pseudomonas was identified by DNA sequencing as the predominant bacterial genus present. The SYBR qPCR tests revealed that the pooled air samples from both the ground and elevated levels had similar total bacterial concentrations, about 1,000 times their culturable ones. Although the endotoxin and (1,3)-beta-d-glucan concentrations were observed to vary over the time, no statistically significant differences were observed in the pooled air samples from the ground and elevated levels over the course of the day. The results from this study elucidated the microbial differences in air at different elevations, and added to the body of evidence of atmospheric mixing of biological agents.; Biology; Environmental Sciences; SCI(E); 18; ARTICLE; 3; 233-244; 26
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/158474]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Li, Kejun,Dong, Shuofei,Wu, Yan,et al. Comparison of the biological content of air samples collected at ground level and at higher elevation[J]. aerobiologia,2010.
APA	Li, Kejun,Dong, Shuofei,Wu, Yan,&Yao, Maosheng.(2010).Comparison of the biological content of air samples collected at ground level and at higher elevation.aerobiologia.
MLA	Li, Kejun,et al."Comparison of the biological content of air samples collected at ground level and at higher elevation".aerobiologia (2010).