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Distribution patterns of denitrification functional genes and microbial floras in multimedia constructed wetlands
Ji, Guodong ; Wang, Rongjing ; Zhi, Wei ; Liu, Xuexin ; Kong, Yaping ; Tan, Yufei
刊名ecological engineering
2012
关键词Constructed wetlands Functional genes Microbial floras Denitrification Distribution patterns WASTE-WATER TREATMENT AMMONIA-OXIDIZING BACTERIA NITROUS-OXIDE REDUCTASE 16S RIBOSOMAL-RNA REAL-TIME PCR NOSZ GENES THIOSPHAERA-PANTOTROPHA DIVERSITY SOILS QUANTIFICATION
DOI10.1016/j.ecoleng.2012.03.015
英文摘要The present study, a quantitative investigation of distribution patterns of functional gene communities, including Anammox bacteria 16S rRNA, amoA, nxrA, narG, napA, nirK, qnorB, nosZ, nas, and nifH in two multimedia constructed wetland systems (CWs)was conducted. DGGE results showed similar distribution patterns in two constructed wetland groups. Lactococcus sp. and Moraxella sp. were the dominant organic nitrogen and denitrification flora; Acinetobacter sp. were the dominant NO3- to NH4+ transformation flora; and Bacillus sp. and beta-Proteobacteria sp. were the dominant NH4+ removal flora in the two constructed wetland groups. Quantitative real-time PCR results showed the qnorB and nas functional genes were predominantly enriched in the 15 cm and 60 cm layers of the CW, which was prepared by multi-media packing of 1% zero-valent iron. The nirK was predominantly enriched in the 60 cm layer of the CW, which was prepared by multi-media packing of 2% zero-valent iron. Other functional nitrogen transformation genes were predominantly enriched in the 15 cm layer of CWs. (C) 2012 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000305443100023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Ecology; Engineering, Environmental; Environmental Sciences; SCI(E); EI; 21; ARTICLE; 179-188; 44
语种英语
内容类型期刊论文
源URL[http://ir.pku.edu.cn/handle/20.500.11897/158375]  
专题环境科学与工程学院
推荐引用方式
GB/T 7714
Ji, Guodong,Wang, Rongjing,Zhi, Wei,et al. Distribution patterns of denitrification functional genes and microbial floras in multimedia constructed wetlands[J]. ecological engineering,2012.
APA Ji, Guodong,Wang, Rongjing,Zhi, Wei,Liu, Xuexin,Kong, Yaping,&Tan, Yufei.(2012).Distribution patterns of denitrification functional genes and microbial floras in multimedia constructed wetlands.ecological engineering.
MLA Ji, Guodong,et al."Distribution patterns of denitrification functional genes and microbial floras in multimedia constructed wetlands".ecological engineering (2012).
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