Negatively and positively charged bacterial aerosol concentration and   diversity in natural environments

doi:10.1007/s11434-013-5852-9

CORC > 北京大学 > 环境科学与工程学院

	Negatively and positively charged bacterial aerosol concentration and diversity in natural environments
	Shen FangXia ; Kai Wei ; Yao MaoSheng
刊名	科学通报英文版
	2013
关键词	positive charge negative charge bacterial aerosol culturable diversity PCR DGGE gel pattern similarity AIRBORNE MICROORGANISMS ELECTROSTATIC PRECIPITATION ELECTRICAL CHARGES SAMPLER BIOAEROSOLS POPULATIONS COLLECTION DEPOSITION PARTICLES INACTIVATION
DOI	10.1007/s11434-013-5852-9
英文摘要	Bioaerosol charge information is of vital importance for their electrostatic collection. Here, electrostatic means and molecular tools were applied to studying bioaerosol charge dynamics. Positively or negatively charged bioaerosols were collected using an electrostatic sampler operated with a field strength of 1.1 kV cm(-1) at a flow rate of 3 L min(-1) for 40 min. Those with fewer or no charges bypassing the sampler were also collected using a filter at the downstream of the electrostatic sampler in one environment. The experiments were independently conducted three times in three different environments. The collected bacterial aerosols were cultured directly on agar plates at 26A degrees C, and the colony forming units (CFU) were manually counted. In addition, the CFUs were washed off from the agar plates, and further subjected to polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) for culturable diversity analysis. The results revealed remarkable differences in positively and negatively charged culturable bacterial aerosol concentration and diversity among the studied environments. In the office environment, negatively charged culturable bacterial aerosols appeared to dominate (P = 0.0489), while in outdoor and hotel environments both polarities had similar concentration levels (P = 0.078, P = 0.88, respectively). DGGE patterns for positively charged culturable bacterial aerosols were shown strikingly different from those of negatively charged regardless of the sampling environments. In addition, for each of the environments positively charged culturable bacterial aerosols collected were found to have more band pattern similarity with those positively charged for respective regions of agar plates than those negatively charged, and vice versa. The information developed here is useful for developing efficient electrostatic sampling protocols for bioaerosols.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000323741500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 2; ARTICLE; 26; 3169-3176; 58
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/158302]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Shen FangXia,Kai Wei,Yao MaoSheng. Negatively and positively charged bacterial aerosol concentration and diversity in natural environments[J]. 科学通报英文版,2013.
APA	Shen FangXia,Kai Wei,&Yao MaoSheng.(2013).Negatively and positively charged bacterial aerosol concentration and diversity in natural environments.科学通报英文版.
MLA	Shen FangXia,et al."Negatively and positively charged bacterial aerosol concentration and diversity in natural environments".科学通报英文版 (2013).