Identification of Retinoic Acid Receptor Agonists in Sewage Treatment   Plants

doi:10.1021/es9000328

CORC > 北京大学 > 城市与环境学院

	Identification of Retinoic Acid Receptor Agonists in Sewage Treatment Plants
	Zhen, Huajun ; Wu, Xiaoqin ; Hu, Jianying ; Xiao, Yang ; Yang, Min ; Hirotsuji, Junji ; Nishikawa, Jun-ichi ; Nakanishi, Tsuyoshi ; Ike, Michihiko
刊名	environmental science technology
	2009
关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY XENOPUS-LAEVIS DEPENDENT TRANSCRIPTION ULTRAVIOLET DETECTION LIMB MALFORMATIONS WASTE-WATER X-RECEPTOR VITAMIN-A DEFORMITIES CHEMICALS
DOI	10.1021/es9000328
英文摘要	Retinoic acid receptor (RAR) agonists are speculated to be one possible cause for the widely observed frog deformities in North America, although little is known about the specific RAR agonists in aquatic environments. We identified the specific RAR agonists in sewage treatment plants (STPs) and receiving rivers using an RAR yeast two-hybrid bioassay. Water samples were extracted by solid-phase extract cartridges, which were successively eluted by hexane, ethyl acetate, and methanol for bioassay. Among the three fractions, the ethyl acetate fraction showed the highest RAR agonistic activities. The bioassay-derived activity, expressed as all-trans-retinoic acid (all-trans-RA) equivalents (ATRA-EQ) were 10.9 +/- 2.2 and 1.7 +/- 1.0 ng/L in the STP influents and effluents, respectively, while the ATRA-EGs were as high as 7.1 and 8.3 ng/L in the two rivers receiving STP effluents. Following a two-step fractionation using high-performance liquid chromatography and ultra-performance liquid chromatography (UPLC) directed by the bioassay,two bioactive fractions were obtained from Gaobeidian STP influent and all-trans-4-oxo-RA (4.7-10.4 ng/L in influents, <0.2-0.9 ng/L in effluents) and 13-cis-4-oxo-RA (2.3-7.1 ng/L in influents, <0.4-1.1 ng/L in effluents) were identified in these fractions with UPLC-MS/MS. The EC(50) for all-trans-4-oxo-RA or 13-cis-4-oxo-RA relative to that of all-trans-RA in exhibiting RAR alpha agonistic activity was calculated to be 3.87 and 0.46, respectively.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000269258000032&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Environmental; Environmental Sciences; SCI(E); EI; PubMed; 21; ARTICLE; 17; 6611-6616; 43
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/160712]
专题	城市与环境学院
推荐引用方式 GB/T 7714	Zhen, Huajun,Wu, Xiaoqin,Hu, Jianying,et al. Identification of Retinoic Acid Receptor Agonists in Sewage Treatment Plants[J]. environmental science technology,2009.
APA	Zhen, Huajun.,Wu, Xiaoqin.,Hu, Jianying.,Xiao, Yang.,Yang, Min.,...&Ike, Michihiko.(2009).Identification of Retinoic Acid Receptor Agonists in Sewage Treatment Plants.environmental science technology.
MLA	Zhen, Huajun,et al."Identification of Retinoic Acid Receptor Agonists in Sewage Treatment Plants".environmental science technology (2009).