Estrogen agonist/antagonist properties of dibenzyl phthalate (DBzP)   based on in vitro and in vivo assays

doi:10.1016/j.toxlet.2011.08.017

CORC > 北京大学 > 城市与环境学院

	Estrogen agonist/antagonist properties of dibenzyl phthalate (DBzP) based on in vitro and in vivo assays
	Zhang, Zhaobin ; Hu, Ying ; Zhao, Liang ; Li, Jun ; Bai, Huicheng ; Zhu, Desheng ; Hu, Jianying
刊名	毒理学快报
	2011
关键词	Estrogenicity Anti-estrogenicity Plasticizer Uterotrophic effect Phthalates Endocrine disrupting chemicals ENDOCRINE-DISRUPTING CHEMICALS N-BUTYL PHTHALATE FETAL-RAT TESTIS REPRODUCTIVE DEVELOPMENT EXPOSURE OBESITY HEALTH WILDLIFE RECEPTOR ESTERS
DOI	10.1016/j.toxlet.2011.08.017
英文摘要	The most commonly used phthalates have been banned or restricted for use as plasticizers in toys in some countries because of their endocrine-disrupting properties. Dibenzyl phthalate (DBzP) has been proposed as a possible alternative for the banned/restricted phthalates. In this study, the estrogen agonist/antagonist properties of DBzP were predicted by molecular docking and confirmed by yeast estrogen screen (YES) and immature mouse uterotrophic assays. The YES assay results showed a dose-dependent increase in DBzP estrogen agonist activity from 10(-6) to 10(-4) M, and at concentrations from 1.95 x 10(-6) M to higher, DBzP significantly inhibited the agonist activity of 10(-9) M 17 beta-estradiol (E(2)), inhibiting 10(-9) M E(2) by 74.5% at its maximum effectiveness. The in vivo estrogen agonist/antagonist activities of DBzP were demonstrated in immature mouse uterotrophic assays. The antagonist activity of DBzP inhibited E(2)-induced uterine growth promoted at 40 and 400 mu g/kg bw (body weight) (P < 0.05). In addition, we also analyzed the estrogen agonist/antagonist potentials of benzyl butyl phthalate (BBP) by YES, and found both were weaker than those of DBzP, suggesting DBzP would be more toxic than BBP and should not be used as an alternative plasticizer. (C) 2011 Elsevier Ireland Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000296177600002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Toxicology; SCI(E); PubMed; 9; ARTICLE; 1; 7-11; 207
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/160651]
专题	城市与环境学院
推荐引用方式 GB/T 7714	Zhang, Zhaobin,Hu, Ying,Zhao, Liang,et al. Estrogen agonist/antagonist properties of dibenzyl phthalate (DBzP) based on in vitro and in vivo assays[J]. 毒理学快报,2011.
APA	Zhang, Zhaobin.,Hu, Ying.,Zhao, Liang.,Li, Jun.,Bai, Huicheng.,...&Hu, Jianying.(2011).Estrogen agonist/antagonist properties of dibenzyl phthalate (DBzP) based on in vitro and in vivo assays.毒理学快报.
MLA	Zhang, Zhaobin,et al."Estrogen agonist/antagonist properties of dibenzyl phthalate (DBzP) based on in vitro and in vivo assays".毒理学快报 (2011).