Molecular and physiological characterization of fluoroquinolone   resistance in relation to uropathogenicity among Escherichia coli   isolates isolated from Wenyu River, China

doi:10.1016/j.chemosphere.2011.11.050

CORC > 北京大学 > 城市与环境学院

	Molecular and physiological characterization of fluoroquinolone resistance in relation to uropathogenicity among Escherichia coli isolates isolated from Wenyu River, China
	Sun, Jianxian ; Hu, Jianying ; Peng, Hui ; Shi, Jiachen ; Dong, Zhaomin
刊名	chemosphere
	2012
关键词	Fluoroquinolone Escherichia coli Resistance Mutation points Uropathogenic Virulence factors URINARY-TRACT-INFECTIONS ANTIBIOTIC-RESISTANCE WASTE-WATER DNA GYRASE ANTIMICROBIAL SUSCEPTIBILITY QUINOLONE RESISTANCE DETERMINING REGION MASS-SPECTROMETRY VIRULENCE FACTORS CIPROFLOXACIN
DOI	10.1016/j.chemosphere.2011.11.050
英文摘要	Increasing antibacterial resistance and pathogenicity in the environment is of growing concern due to its potential human risk. In the present study, 236 Escherichia coli isolates were collected from Wenyu River in China on drugless (48 isolates) and quinolone-containing plates (189 isolates). Their minimum inhibitory concentrations (MICs) were determined ranging from 0.125 mu g mL(-1) to 128 mu g mL(-1). Mutation points related to fluoroquinolone resistance were observed at S83 to L and D87 to N or Y in the GyrA subunit and 580 to Rot I and E84 to G in the ParC subunit. Generally, MICs of LEV and GAT are dependent on the patterns of these mutation points. The profile with three mutation points was related to LEV-resistant E. coli isolates, and the (S83L, D87N + S80I) mutation profile was most prevalent (65.7%) in LEV-resistant isolates, while a large proportion of isolates, even those with three mutation points, were susceptive to GAT. The incidence of virulence factors in LEV-resistant isolates (44.7%, 59/132) was much higher than in nonresistant isolates (23.1%, 24/104) (chi(2) = 11.925, 1 degrees of freedom, p < 0.001) indicating that fluoroquinolone-resistant E. coli would pose a potential risk. A similar distribution was also found in isolates resistant to GAT (chi(2) = 7.843, 1 degrees of freedom, p = 0.0079). (C) 2011 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000301686000007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Environmental Sciences; SCI(E); EI; PubMed; 8; ARTICLE; 1; 37-42; 87
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/160650]
专题	城市与环境学院
推荐引用方式 GB/T 7714	Sun, Jianxian,Hu, Jianying,Peng, Hui,et al. Molecular and physiological characterization of fluoroquinolone resistance in relation to uropathogenicity among Escherichia coli isolates isolated from Wenyu River, China[J]. chemosphere,2012.
APA	Sun, Jianxian,Hu, Jianying,Peng, Hui,Shi, Jiachen,&Dong, Zhaomin.(2012).Molecular and physiological characterization of fluoroquinolone resistance in relation to uropathogenicity among Escherichia coli isolates isolated from Wenyu River, China.chemosphere.
MLA	Sun, Jianxian,et al."Molecular and physiological characterization of fluoroquinolone resistance in relation to uropathogenicity among Escherichia coli isolates isolated from Wenyu River, China".chemosphere (2012).