Embryonic hematopoiesis in vertebrate somites gives rise to definitive   hematopoietic stem cells

doi:10.1093/jmcb/mjw024

CORC > 北京大学 > 生命科学学院

	Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells
	Qiu, Juhui ; Fan, Xiaoying ; Wang, Yixia ; Jin, Hongbin ; Song, Yixiao ; Han, Yang ; Huang, Shenghong ; Meng, Yaping ; Tang, Fuchou ; Meng, Anming
刊名	JOURNAL OF MOLECULAR CELL BIOLOGY
	2016
关键词	primitive hematopoiesis definitive hematopoiesis stem cells blood somite embryos zebrafish SMOOTH-MUSCLE-CELLS TRANSGENIC ZEBRAFISH AORTIC ENDOTHELIUM BLOOD ORIGIN TRANSITION MIGRATION LINEAGES PROTEIN MOUSE
DOI	10.1093/jmcb/mjw024
英文摘要	Hematopoietic stem cells (HSCs) replenish all types of blood cells. It is debating whether HSCs in adults solely originate from the aorta-gonad-mesonephros (AGM) region, more specifically, the dorsal aorta, during embryogenesis. Here, we report that somite hematopoiesis, a previously unwitnessed hematopoiesis, can generate definitive HSCs (dHSCs) in zebrafish. By transgenic lineage tracing, we found that a subset of cells within the forming somites emigrate ventromedially and mix with lateral plate mesoderm-derived primitive hematopoietic cells before the blood circulation starts. These somite-derived hematopoietic precursors and stem cells (sHPSCs) subsequently enter the circulation and colonize the kidney of larvae and adults. RNA-seq analysis reveals that sHPSCs express hematopoietic genes with sustained expression of many muscle/skeletal genes. Embryonic sHPSCs transplanted into wild-type embryos expand during growth and survive for life time with differentiation into various hematopoietic lineages, indicating self-renewal and multipotency features. Therefore, the embryonic origin of dHSCs in adults is not restricted to the AGM.; Major Science Programs of MOST of China [2012CB945101, 2011CB943800]; National Natural Science Foundation of China [31221064]; China Postdoctoral Science Foundation [2013M540931]; Tsinghua University Initiative Scientific Research Program [20141081299]; SCI(E); PubMed; ARTICLE; jhqiu2008@126.com; mengam@mail.tsinghua.edu.cn; 4; 288-301; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/493643]
专题	生命科学学院
推荐引用方式 GB/T 7714	Qiu, Juhui,Fan, Xiaoying,Wang, Yixia,et al. Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2016.
APA	Qiu, Juhui.,Fan, Xiaoying.,Wang, Yixia.,Jin, Hongbin.,Song, Yixiao.,...&Meng, Anming.(2016).Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells.JOURNAL OF MOLECULAR CELL BIOLOGY.
MLA	Qiu, Juhui,et al."Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells".JOURNAL OF MOLECULAR CELL BIOLOGY (2016).