Development of the Double Cyclic Peptide Ligand for Antibody   Purification and Protein Detection

doi:10.1021/acs.bioconjchem.6b00170

CORC > 北京大学 > 生命科学学院

	Development of the Double Cyclic Peptide Ligand for Antibody Purification and Protein Detection
	Gong, Yiyi ; Zhang, Lin ; Li, Jin ; Feng, Shan ; Deng, Haiteng
刊名	BIOCONJUGATE CHEMISTRY
	2016
关键词	PHAGE DISPLAY EXPRESSION BINDING DESIGN PCNA
DOI	10.1021/acs.bioconjchem.6b00170
英文摘要	Development of a peptide-based affinity matrix and detection reagent is important for biomedical research and the biopharmaceutical industry. In the present work, we designed and synthesized an immunoglobin G (IgG)-binding peptide ligand, Fc-III-4C. Fc-III-4C is composed of 15 residues, and the 4 cysteine residues form 2 disulfide bonds to generate a double cyclic structure. The binding affinity of the Fc-III-4C peptide toward human IgG was determined to be 2.45 nIvI (K-d), which is higher than that of IgG with Protein A/G (Pro-A/G). Importantly, the Fc-III-4C peptide displayed high affinity to various IgGs from different species. Fc-III-4C immobilized agarose beads exhibited high stability and reusability when compared with that of the Pro-A/G-immobilized beads. The conjugate of Fc-III-4C with FITC was demonstrated to be suitable for immunofluorescence detection of proteins expressed in cells. These results demonstrate that the Fc-III-4C peptide is a useful affinity ligand for antibody purification and as a protein detection reagent.; NSFC [31270871, 21502103]; MOEC [2012Z02293]; Chinese Ministry of Science and Technology [2014CBA02005, 2014AA020907]; Global Science Alliance Pro-Gram of Thermo-Fisher Scientific; SCI(E); PubMed; ARTICLE; fengs@mail.tsinghua.edu.cn; dht@mil.tsinghua.edu.cn; 7; 1569-1573; 27
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/492020]
专题	生命科学学院
推荐引用方式 GB/T 7714	Gong, Yiyi,Zhang, Lin,Li, Jin,et al. Development of the Double Cyclic Peptide Ligand for Antibody Purification and Protein Detection[J]. BIOCONJUGATE CHEMISTRY,2016.
APA	Gong, Yiyi,Zhang, Lin,Li, Jin,Feng, Shan,&Deng, Haiteng.(2016).Development of the Double Cyclic Peptide Ligand for Antibody Purification and Protein Detection.BIOCONJUGATE CHEMISTRY.
MLA	Gong, Yiyi,et al."Development of the Double Cyclic Peptide Ligand for Antibody Purification and Protein Detection".BIOCONJUGATE CHEMISTRY (2016).