Deep sequencing reveals global patterns of mRNA recruitment during   translation initiation

doi:10.1038/srep30170

CORC > 北京大学 > 生命科学学院

	Deep sequencing reveals global patterns of mRNA recruitment during translation initiation
	Gao, Rong ; Yu, Kai ; Nie, Jukui ; Lian, Tengfei ; Jin, Jianshi ; Liljas, Anders ; Su, Xiao-Dong
刊名	SCIENTIFIC REPORTS
	2016
关键词	RIBOSOMAL-PROTEIN S1 ESCHERICHIA-COLI SECONDARY STRUCTURE GENE-EXPRESSION BACTERIA SPECIFICITY MECHANISMS SELECTION COMPLEX SITES
DOI	10.1038/srep30170
英文摘要	In this work, we developed a method to systematically study the sequence preference of mRNAs during translation initiation. Traditionally, the dynamic process of translation initiation has been studied at the single molecule level with limited sequencing possibility. Using deep sequencing techniques, we identified the sequence preference at different stages of the initiation complexes. Our results provide a comprehensive and dynamic view of the initiation elements in the translation initiation region (TIR), including the S1 binding sequence, the Shine-Dalgarno (SD)/anti-SD interaction and the second codon, at the equilibrium of different initiation complexes. Moreover, our experiments reveal the conformational changes and regional dynamics throughout the dynamic process of mRNA recruitment.; National Natural Science Foundation of China [31170710, 31270803]; National Basic Research Program of China (973 Program) [2011CB911103]; Peking University 985 grant; SCI(E); PubMed; ARTICLE; gaorong@pku.edu.cn; xdsu@pku.edu.cn; 30170; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/491833]
专题	生命科学学院
推荐引用方式 GB/T 7714	Gao, Rong,Yu, Kai,Nie, Jukui,et al. Deep sequencing reveals global patterns of mRNA recruitment during translation initiation[J]. SCIENTIFIC REPORTS,2016.
APA	Gao, Rong.,Yu, Kai.,Nie, Jukui.,Lian, Tengfei.,Jin, Jianshi.,...&Su, Xiao-Dong.(2016).Deep sequencing reveals global patterns of mRNA recruitment during translation initiation.SCIENTIFIC REPORTS.
MLA	Gao, Rong,et al."Deep sequencing reveals global patterns of mRNA recruitment during translation initiation".SCIENTIFIC REPORTS (2016).