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	Identification of EFHD1 as a novel Ca~(2+) sensor for mitoflash activation; Identification of EFHD1 as a novel Ca~(2+) sensor for mitoflash activation
	Tingting Hou ; Chongshu Jian ; Jiejia Xu ; Jianzhong Xi ; Keping Hu ; Liping Wei ; Heping Cheng ; Xianhua Wang
	2016
关键词	mitochondrial screening superoxide respiration disruption targeted accompanied alter burst sought mitochondrial screening superoxide respiration disruption targeted accompanied alter burst sought
英文摘要	Mitochondrial flashes(mitoflashes) represent stochastic and discrete mitochondrial events that each comprises a burst of superoxide production accompanied by transient depolarization and matrix alkalinization in a respiratory mitochondrion. While mitochondrial Ca~(2+) is shown to be an important regulator of mitoflash activity, little is known about its specific mechanism of action. Here we sought to determine possible molecular players that mediate the Ca~(2+) regulation of mitoflashes by screening mitochondrial proteins containing the Ca~(2+)-binding motifs. In silico analysis and targeted si RNA screening identified four mitoflash activators(MICU1, EFHD1, SLC25A23, SLC25A25) and one mitoflash inhibitor(LETM1) in terms of their ability to modulate mitoflash response to hyperosmotic stress. In particular, overexpression or downregulation of EFHD1 enhanced or depressed mitoflash activation, respectively, under various conditions of mitochondrial Ca~(2+) elevations. Yet, it did not alter mitochondrial Ca~(2+) ...; Mitochondrial flashes(mitoflashes) represent stochastic and discrete mitochondrial events that each comprises a burst of superoxide production accompanied by transient depolarization and matrix alkalinization in a respiratory mitochondrion. While mitochondrial Ca~(2+) is shown to be an important regulator of mitoflash activity, little is known about its specific mechanism of action. Here we sought to determine possible molecular players that mediate the Ca~(2+) regulation of mitoflashes by screening mitoch; 中国生物物理学会神经生物物理与神经信息学专业委员会; 1
语种	英语
出处	第十一届全国钙信号和细胞功能研讨会
内容类型	其他
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/479936]
专题	生命科学学院 工学院
推荐引用方式 GB/T 7714	Tingting Hou,Chongshu Jian,Jiejia Xu,et al. Identification of EFHD1 as a novel Ca~(2+) sensor for mitoflash activation, Identification of EFHD1 as a novel Ca~(2+) sensor for mitoflash activation. 2016-01-01.

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