Generation of Homogeneous PDX1~+ Pancreatic Progenitors from Human ES Cell-derived Endoderm Cells

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	Generation of Homogeneous PDX1~+ Pancreatic Progenitors from Human ES Cell-derived Endoderm Cells
	Jun Cai ; Chen Yu ; Liu Yanxia ; Chen Song ; Yuxuan Guo ; Jun Yong ; Lu Wei ; Mingxiao Ding ; Hongkui Deng
刊名	Journal of Molecular Cell Biology
	2010
关键词	embryonic stem cell retinoic acid PDX1+ pancreatic progenitor hepatic Smad
英文摘要	One key step in producing insulin-secreting cells from human embryonic stem (hES) cells is the generation of pancreatic and duodenal homeobox gene 1 (PDX1)-expressing pancreatic progenitor cells. All-trans retinoic acid (RA) has important roles in pancreas development and is widely used to induce pancreatic differentiation of ES cells. When RA was added directly to the activin A-induced hES cells, >20% cells were positive for the pancreatic marker PDX1, whereas the other cells were mainly hepatic cells. We found that when the activin A-induced hES cells were replated and seeded at low cell densities, the addition of RA induced significant pancreatic differentiation and over 70%of cells in culture expressed PDX1. When the endodermal cells were isolated with the surface marker CXCR4 from the activin A-induced culture and further differentiated with RA, a homogeneous PDX1~+ cell population (over 95% pure) was generated. The PDX1~+ cells could further differentiate into cells that expressed pancreatic transcription factors and pancreatic endocrine or exocrine markers. We also found that RA inhibited the hepatic differentiation of endodermal cells that were seeded at low cell densities, and this inhibition may have been through the inhibition of Smad1/5/8 activity. Thus, we present a highly efficient and reproducible protocol for generating PDX11 pancreatic progenitor cells from hES cells.; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 1; 50-60; 2
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/478946]
专题	生命科学学院
推荐引用方式 GB/T 7714	Jun Cai,Chen Yu,Liu Yanxia,et al. Generation of Homogeneous PDX1~+ Pancreatic Progenitors from Human ES Cell-derived Endoderm Cells[J]. Journal of Molecular Cell Biology,2010.
APA	Jun Cai.,Chen Yu.,Liu Yanxia.,Chen Song.,Yuxuan Guo.,...&Hongkui Deng.(2010).Generation of Homogeneous PDX1~+ Pancreatic Progenitors from Human ES Cell-derived Endoderm Cells.Journal of Molecular Cell Biology.
MLA	Jun Cai,et al."Generation of Homogeneous PDX1~+ Pancreatic Progenitors from Human ES Cell-derived Endoderm Cells".Journal of Molecular Cell Biology (2010).