Engineering Escherichia coli to bind to cyanobacteria | |
Zhang, Zijian ; Meng, Liuyi ; Ni, Congjian ; Yao, Lanqiu ; Zhang, Fengyu ; Jin, Yuji ; Mu, Xuelang ; Zhu, Shiyu ; Lu, Xiaoyu ; Liu, Shiyu ; Yu, Congyu ; Wang, Chenggong ; Zheng, Pu ; Wu, Jie ; Kang, Li ; Zhang, Haoqian M. ; Ouyang, Qi | |
刊名 | JOURNAL OF BIOSCIENCE AND BIOENGINEERING |
2017 | |
关键词 | Surface display Lectin Microvirin Binding Cyanobacteria Escherichia coil LECTIN MICROCYSTINS NUTRITION |
DOI | 10.1016/jpiosc.2016.09.010 |
英文摘要 | We engineered Escherichia coli cells to bind to cyanobacteria by heterologously producing and displaying lectins of the target cyanobacteria on their surface. To prove the efficacy of our approach, we tested this design on Micro cystis aeruginosa with microvirin (Mvn), the lectin endogenously produced by this cyanobacterium. The coding sequence of Mvn was C-terminally fused to the ice nucleation protein NC (INPNC) gene and expressed in E. coll. Results showed that E. colt cells expressing the INPNC::Mvn fusion protein were able to bind to M. aeruginosa and the average number of E. colt cells bound to each cyanobacterial cell was enhanced 8-fold. Finally, a computational model was developed to simulate the binding reaction and help reconstruct the binding parameters. To our best knowledge, this is the first report on the binding of two organisms in liquid culture mediated by the surface display of lectins and it may serve as a novel approach to mediate microbial adhesion. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.; Peking University; Fund for Fostering Talents in Basic Science of the National Natural Science Foundation of China [J1310033]; SCI(E); ARTICLE; 3; 347-352; 123 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/474853] |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Zhang, Zijian,Meng, Liuyi,Ni, Congjian,et al. Engineering Escherichia coli to bind to cyanobacteria[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2017. |
APA | Zhang, Zijian.,Meng, Liuyi.,Ni, Congjian.,Yao, Lanqiu.,Zhang, Fengyu.,...&Ouyang, Qi.(2017).Engineering Escherichia coli to bind to cyanobacteria.JOURNAL OF BIOSCIENCE AND BIOENGINEERING. |
MLA | Zhang, Zijian,et al."Engineering Escherichia coli to bind to cyanobacteria".JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2017). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论