Engineering the ribosomal DNA in a megabase synthetic chromosome

doi:10.1126/science.aaf3981

CORC > 北京大学 > 生命科学学院

	Engineering the ribosomal DNA in a megabase synthetic chromosome
	Zhang, Weimin ; Zhao, Guanghou ; Luo, Zhouqing ; Lin, Yicong ; Wang, Lihui ; Guo, Yakun ; Wang, Ann ; Jiang, Shuangying ; Jiang, Qingwen ; Gong, Jianhui ; Wang, Yun ; Hou, Sha ; Huang, Jing ; Li, Tianyi ; Qin, Yiran ; Dong, Junkai ; Qin, Qin ; Zhang, Jiaying ; Zou, Xinzhi ; He, Xi ; Zhao, Li ; Xiao, Yibo ; Xu, Meng ; Cheng, Erchao ; Huang, Ning ; Zhou, Tong ; Shen, Yue ; Walker, Roy ; Luo, Yisha ; Kuang, Zheng ; Mitchell, Leslie A. ; Yang, Kun ; Richardson, Sarah M. ; Wu, Yi ; Li, Bing-Zhi ; Yuan, Ying-Jin ; Yang, Huanming ; Lin, Jiwei ; Chen, Guo-Qiang ; Wu, Qingyu ; Bader, Joel S. ; Ca
刊名	SCIENCE
	2017
关键词	SACCHAROMYCES-CEREVISIAE MOLECULAR-GENETICS YEAST GENES NUCLEOLUS SEQUENCE DESIGN
DOI	10.1126/science.aaf3981
英文摘要	We designed and synthesized a 976,067-base pair linear chromosome, synXII, based on native chromosome XII in Saccharomyces cerevisiae. SynXII was assembled using a twostep method, specified by successive megachunk integration and meiotic recombinationmediated assembly, producing a functional chromosome in S. cerevisiae. Minor growth defect " bugs" detected in synXII, caused by deletion of tRNA genes, were rescued by introducing an ectopic copy of a single tRNA gene. The ribosomal gene cluster (rDNA) on synXII was left intact during the assembly process and subsequently replaced by a modified rDNA unit used to regenerate rDNA at three distinct chromosomal locations. The signature sequences within rDNA, which can be used to determine species identity, were swapped to generate a Saccharomyces synXII strain that would be identified as Saccharomyces bayanus by standard DNA barcoding procedures.; National Natural Science Foundation of China [31471254, 21621004, 21390203]; Chinese Ministry of Science and Technology [2012CB725201, 2012AA02A708]; Ph.D. Programs Foundation of Ministry of Education of China [20110002120055]; Research Fund for the Doctoral Program of Higher Education of China [20120002110022]; Tsinghua University Initiative grant [2011Z02296]; NSF [MCB-1026068, MCB-1158201, MCB-1445545]; SCI(E); ARTICLE; 6329,SI; 355
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474628]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, Weimin,Zhao, Guanghou,Luo, Zhouqing,et al. Engineering the ribosomal DNA in a megabase synthetic chromosome[J]. SCIENCE,2017.
APA	Zhang, Weimin.,Zhao, Guanghou.,Luo, Zhouqing.,Lin, Yicong.,Wang, Lihui.,...&Ca.(2017).Engineering the ribosomal DNA in a megabase synthetic chromosome.SCIENCE.
MLA	Zhang, Weimin,et al."Engineering the ribosomal DNA in a megabase synthetic chromosome".SCIENCE (2017).