MagR Alone Is Insufficient to Confer Cellular Calcium Responses to   Magnetic Stimulation

doi:10.3389/fncir.2017.00011

CORC > 北京大学 > 生命科学学院

	MagR Alone Is Insufficient to Confer Cellular Calcium Responses to Magnetic Stimulation
	Pang, Keliang ; You, He ; Chen, Yanbo ; Chu, Pengcheng ; Hu, Meiqin ; Shen, Jianying ; Guo, Wei ; Xie, Can ; Lu, Bai
刊名	FRONTIERS IN NEURAL CIRCUITS
	2017
关键词	magnetic field calcium imaging hippocampal neurons neural modulation cryptochrome magnetogenetics NEURAL ACTIVITY
DOI	10.3389/fncir.2017.00011
英文摘要	Magnetic manipulation of cell activity offers advantages over optical manipulation but an ideal tool remains elusive. The MagR protein was found through its interaction with cryptochrome (Cry) and the protein in solution appeared to respond to magnetic stimulation (MS). After we initiated an investigation on the specific role of MagR in cellular response to MS, a subsequent study claimed that MagR expression alone could achieve cellular activation by MS. Here we report that despite systematically testing different ways of measuring intracellular calcium and different MS protocols, it was not possible to detect any cellular or neuronal responses to MS in MagR-expressing HEK cells or primary neurons from the dorsal root ganglion and the hippocampus. By contrast, in neurons co-expressing MagR and channelrhodopin, optical but not MS increased calcium influx in hippocampal neurons. Our results indicate that MagR alone is not sufficient to confer cellular magnetic responses.; Start-up funds of the Tsinghua University; SCI(E); ARTICLE; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474558]
专题	生命科学学院
推荐引用方式 GB/T 7714	Pang, Keliang,You, He,Chen, Yanbo,et al. MagR Alone Is Insufficient to Confer Cellular Calcium Responses to Magnetic Stimulation[J]. FRONTIERS IN NEURAL CIRCUITS,2017.
APA	Pang, Keliang.,You, He.,Chen, Yanbo.,Chu, Pengcheng.,Hu, Meiqin.,...&Lu, Bai.(2017).MagR Alone Is Insufficient to Confer Cellular Calcium Responses to Magnetic Stimulation.FRONTIERS IN NEURAL CIRCUITS.
MLA	Pang, Keliang,et al."MagR Alone Is Insufficient to Confer Cellular Calcium Responses to Magnetic Stimulation".FRONTIERS IN NEURAL CIRCUITS (2017).