Single-cell whole-genome analyses by Linear Amplification via Transposon   Insertion (LIANTI)

doi:10.1126/science.aak9787

CORC > 北京大学 > 生命科学学院

	Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI)
	Chen, Chongyi ; Xing, Dong ; Tan, Longzhi ; Li, Heng ; Zhou, Guangyu ; Huang, Lei ; Xie, X. Sunney
刊名	SCIENCE
	2017
关键词	MULTIPLE DISPLACEMENT AMPLIFICATION DNA-DAMAGE CHROMATIN ORGANIZATION EXCISION-REPAIR MUTATION-RATES CANCER TRANSCRIPTION NUCLEOTIDE STRAND RECOMBINATION
DOI	10.1126/science.aak9787
英文摘要	Single-cell genomics is important for biology and medicine. However, current whole-genome amplification (WGA) methods are limited by low accuracy of copy-number variation (CNV) detection and low amplification fidelity. Here we report an improved single-cell WGA method, Linear Amplification via Transposon Insertion (LIANTI), which outperforms existing methods, enabling micro-CNV detection with kilobase resolution. This allowed direct observation of stochastic firing of DNA replication origins, which differs from cell to cell. We also show that the predominant cytosine-to-thymine mutations observed in single-cell genomics often arise from the artifact of cytosine deamination upon cell lysis. However, identifying single-nucleotide variations (SNVs) can be accomplished by sequencing kindred cells. We determined the spectrum of SNVs in a single human cell after ultraviolet radiation, revealing their nonrandom genome-wide distribution.; NIH [5DP1CA186693]; National Cancer Institute [5R33CA174560]; Beijing Municipal Science and Technology Commission [D1511000024150002]; National Key Technologies Research and Development program [2016YFC0900100]; Beijing Advanced Innovation Center for Genomics at Peking University; Howard Hughes Medical Institute; SCI(E); ARTICLE; 6334; 189-194; 356
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474005]
专题	生命科学学院
推荐引用方式 GB/T 7714	Chen, Chongyi,Xing, Dong,Tan, Longzhi,et al. Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI)[J]. SCIENCE,2017.
APA	Chen, Chongyi.,Xing, Dong.,Tan, Longzhi.,Li, Heng.,Zhou, Guangyu.,...&Xie, X. Sunney.(2017).Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI).SCIENCE.
MLA	Chen, Chongyi,et al."Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI)".SCIENCE (2017).