Chemoproteomic Profiling of Bile Acid Interacting Proteins

doi:10.1021/acscentsci.7b00134

CORC > 北京大学 > 生命科学学院

	Chemoproteomic Profiling of Bile Acid Interacting Proteins
	Zhuang, Shentian ; Li, Qiang ; Cai, Lirong ; Wang, Chu ; Lei, Xiaoguang
刊名	ACS CENTRAL SCIENCE
	2017
关键词	NONALCOHOLIC-FATTY-LIVER FARNESOID-X-RECEPTOR NUCLEAR RECEPTOR TAUROURSODEOXYCHOLIC ACID ALZHEIMERS-DISEASE EMERGING ROLE OBETICHOLIC ACID BINDING-PROTEINS VIBRIO-CHOLERAE HOST METABOLISM
DOI	10.1021/acscentsci.7b00134
英文摘要	Bile acids (BAs) are a family of endogenous metabolites synthesized from cholesterol in liver and modified by microbiota in gut. Being amphipathic molecules, the major function of BAs is to help with dietary lipid digestion. In addition, they also act as signaling molecules to regulate lipid and glucose, metabolism as well as gut microbiota composition in the host. Remarkably, recent discoveries of the dedicated receptors for BAs such as FXR and TGRS have uncovered a number of novel actions of BAs as signaling hormones which play significant roles in both physiological and pathological conditions. Disorders in BAs' metabolism are closely related to metabolic syndrome and intestinal and neurodegenerative diseases. Though BA-based therapies have been clinically implemented for decades, the regulatory mechanism of BA is still poorly understood and a comprehensive characterization of BA-interacting proteins in proteome remains elusive. We herein describe a chemoproteomic strategy that uses a number of structurally diverse, clickable, and photoreactive BA-based probes in combination with quantitative mass spectrometry to globally profile BA-interacting proteins in mammalian, cells. Over 600 BA-interacting protein targets were identified, including known endogenous receptors and transporters of BA. Analysis of these novel BA-interacting proteins revealed that they are mainly enriched in functional pathways such as endoplasmic reticulum (ER) stress response and lipid metabolism, and are predicted with strong implications with Alzheimer's disease, non-alcoholic fatty liver disease, and diarrhea. Our findings will significantly improve the current understanding of BAs' regulatory roles in human physiology and diseases.; NNSFC [21472010, 21521003, 21561142002, 21625201, 21472008, 81490741]; Ministry of Science and Technology of China [2015CB856200, 2016YFA0501500]; "1000 Talents Plan" Young Investigator Award; SCI(E); ARTICLE; 5; 501-509; 3
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/473639]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhuang, Shentian,Li, Qiang,Cai, Lirong,et al. Chemoproteomic Profiling of Bile Acid Interacting Proteins[J]. ACS CENTRAL SCIENCE,2017.
APA	Zhuang, Shentian,Li, Qiang,Cai, Lirong,Wang, Chu,&Lei, Xiaoguang.(2017).Chemoproteomic Profiling of Bile Acid Interacting Proteins.ACS CENTRAL SCIENCE.
MLA	Zhuang, Shentian,et al."Chemoproteomic Profiling of Bile Acid Interacting Proteins".ACS CENTRAL SCIENCE (2017).