Four Closely-related RING-type E3 Ligases, APD1-4, are Involved in Pollen Mitosis II Regulation in Arabidopsis

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	Four Closely-related RING-type E3 Ligases, APD1-4, are Involved in Pollen Mitosis II Regulation in Arabidopsis
	Luo Guo ; Gu Hongya ; Liu Jingjing ; Qu Lijia
刊名	Journal of Integrative Plant Biology
	2012
关键词	Male gametogenesis pollen mitosis II RING-finger E3 ligase Arabidopsis
英文摘要	Ubiquitination of proteins is one of the critical regulatory mechanisms in eukaryotes. In higher plants, protein ubiquitination plays an essential role in many biological processes, including hormone signaling, photomorphogenesis, and pathogen defense. However, the roles of protein ubiquitination in the reproductive process are not clear. In this study, we identified four plant-specific RING-finger genes designated Aberrant Pollen Development 1_ (APD1) to APD4, as regulators of pollen mitosis II (PMII) in Arabidopsis thafiana (L.). The apdl apd2 double mutant showed a significantly increased percentage of bicellular-like pollen at the mature pollen stage. Further downregulation of the APD3 and APD4 transcripts in apdl apd2 by RNA interference (RNAi) resulted in more severe abnormal bicellular-like pollen phenotypes than in apdl apd2, suggesting that cell division was defective in male gametogenesis. All of the four genes were expressed in multiple stages at different levels during male gametophyte development. Confocal analysis using green florescence fusion proteins (GFP) GFP-APD1 and GFP-APD2 showed that APDs are associated with intracellular membranes. Furthermore, APD2 had E2-dependent E3 ligase activity in vitro, and five APD2-interacting proteins were identified. Our results suggest that these four genes may be involved, redundantly, in regulating the PMII process during male gametogenesis.; the National Key Basic Research Program of the People's Republic of China,the National Science Foundation of China; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 10; 814-827; 54
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/462143]
专题	生命科学学院
推荐引用方式 GB/T 7714	Luo Guo,Gu Hongya,Liu Jingjing,et al. Four Closely-related RING-type E3 Ligases, APD1-4, are Involved in Pollen Mitosis II Regulation in Arabidopsis[J]. Journal of Integrative Plant Biology,2012.
APA	Luo Guo,Gu Hongya,Liu Jingjing,&Qu Lijia.(2012).Four Closely-related RING-type E3 Ligases, APD1-4, are Involved in Pollen Mitosis II Regulation in Arabidopsis.Journal of Integrative Plant Biology.
MLA	Luo Guo,et al."Four Closely-related RING-type E3 Ligases, APD1-4, are Involved in Pollen Mitosis II Regulation in Arabidopsis".Journal of Integrative Plant Biology (2012).