Single-cell triple omics sequencing reveals genetic, epigenetic, and   transcriptomic heterogeneity in hepatocellular carcinomas

doi:10.1038/cr.2016.23

CORC > 北京大学 > 生命科学学院

	Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas
	Hou, Yu ; Guo, Huahu ; Cao, Chen ; Li, Xianlong ; Hu, Boqiang ; Zhu, Ping ; Wu, Xinglong ; Wen, Lu ; Tang, Fuchou ; Huang, Yanyi ; Peng, Jirun
刊名	CELL RESEARCH
	2016
关键词	scTrio-seq CNV transcriptome DNA methylome HCC DNA METHYLATION LANDSCAPE CIRCULATING TUMOR-CELLS COPY NUMBER VARIATION EMBRYONIC STEM-CELLS MESSENGER-RNA-SEQ COMPLEMENT ACTIVATION CANCER EPIGENETICS LUNG-CANCER GENOME EXPRESSION
DOI	10.1038/cr.2016.23
英文摘要	Single-cell genome, DNA methylome, and transcriptome sequencing methods have been separately developed. However, to accurately analyze the mechanism by which transcriptome, genome and DNA methylome regulate each other, these omic methods need to be performed in the same single cell. Here we demonstrate a single-cell triple omics sequencing technique, scTrio-seq, that can be used to simultaneously analyze the genomic copy-number variations (CNVs), DNA methylome, and transcriptome of an individual mammalian cell. We show that large-scale CNVs cause proportional changes in RNA expression of genes within the gained or lost genomic regions, whereas these CNVs generally do not affect DNA methylation in these regions. Furthermore, we applied scTrio-seq to 25 single cancer cells derived from a human hepatocellular carcinoma tissue sample. We identified two subpopulations within these cells based on CNVs, DNA methylome, or transcriptome of individual cells. Our work offers a new avenue of dissecting the complex contribution of genomic and epigenomic heterogeneities to the transcriptomic heterogeneity within a population of cells.; supported by grants from the National Natural Science Foundation of China,the Ministry of Sciences and Technology of China; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); ARTICLE; tangfuchou@pku.edu.cn; yanyi@pku.edu.cn; pengjr@medmail.com.cn; 3; 304-319; 26
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/449927]
专题	生命科学学院工学院
推荐引用方式 GB/T 7714	Hou, Yu,Guo, Huahu,Cao, Chen,et al. Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas[J]. CELL RESEARCH,2016.
APA	Hou, Yu.,Guo, Huahu.,Cao, Chen.,Li, Xianlong.,Hu, Boqiang.,...&Peng, Jirun.(2016).Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas.CELL RESEARCH.
MLA	Hou, Yu,et al."Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas".CELL RESEARCH (2016).